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Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor
State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Laboratoire IMAGES EA 4218, Groupe Biocapteurs, Université de Perpignan Via Domitia (UPVD), Bâtiment S, 52 av Paul Alduy 66860 Perpignan Cedex, France
* Author to whom correspondence should be addressed.
Received: 11 March 2013; in revised form: 3 April 2013 / Accepted: 7 April 2013 / Published: 15 April 2013
Abstract: Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H2O2) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H2O2, applying a potential of +0.6 V, +0.6 V, +0.4 V, −0.2 V and −0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H2O2 were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H2O2 at a working potential as low as −0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.
Keywords: methyl mercaptan; hydrogen peroxide; amperometric sensor; screen printed electrode
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Li, Z.-H.; Guedri, H.; Viguier, B.; Sun, S.-G.; Marty, J.-L. Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor. Sensors 2013, 13, 5028-5039.
Li Z-H, Guedri H, Viguier B, Sun S-G, Marty J-L. Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor. Sensors. 2013; 13(4):5028-5039.
Li, Zhan-Hong; Guedri, Houssemeddine; Viguier, Bruno; Sun, Shi-Gang; Marty, Jean-Louis. 2013. "Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor." Sensors 13, no. 4: 5028-5039.