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Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor
School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China
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Received: 6 February 2013; in revised form: 23 March 2013 / Accepted: 25 March 2013 / Published: 28 March 2013
Abstract: In this study, we have first developed a rapid and sensitive strip immunosensor based on two heterogeneously-sized gold nanoparticles (Au NPs) probes for the detection of trace lead ions in drinking water. The sensitivity was 4-fold higher than that of the conventional LFA under the optimized conditions. The visual limit of detection (LOD) of the amplified method for qualitative detection lead ions was 2 ng/mL and the LOD for semi-quantitative detection could go down to 0.19 ng/mL using a scanning reader. The method suffered from no interference from other metal ions and could be used to detect trace lead ions in drinking water without sample enrichment. The recovery of the test samples ranged from 96% to 103%. As the detection method could be accomplished within 15 min, this method could be used as a potential tool for preliminary monitoring of lead contamination in drinking water.
Keywords: amplified competitive lateral ﬂow assay; lead ions; immunosensor; gold nanoparticles
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MDPI and ACS Style
Kuang, H.; Xing, C.; Hao, C.; Liu, L.; Wang, L.; Xu, C. Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor. Sensors 2013, 13, 4214-4224.
Kuang H, Xing C, Hao C, Liu L, Wang L, Xu C. Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor. Sensors. 2013; 13(4):4214-4224.
Kuang, Hua; Xing, Changrui; Hao, Changlong; Liu, Liqiang; Wang, Libing; Xu, Chuanlai. 2013. "Rapid and Highly Sensitive Detection of Lead Ions in Drinking Water Based on a Strip Immunosensor." Sensors 13, no. 4: 4214-4224.