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Photonic Crystal Biosensor Based on Optical Surface Waves
Institute of Spectroscopy, Russian Academy of Sciences, Fizicheskaya, 5, Troitsk, Moscow Region, 142190, Russia
Laboratoire de Physique de la Matière Vivante, Institut de Physique des Systèmes Biologiques, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland
* Authors to whom correspondence should be addressed.
Received: 11 January 2013; in revised form: 7 February 2013 / Accepted: 8 February 2013 / Published: 19 February 2013
Abstract: A label-free biosensor device based on registration of photonic crystal surface waves is described. Angular interrogation of the optical surface wave resonance is used to detect changes in the thickness of an adsorbed layer, while an additional simultaneous detection of the critical angle of total internal reflection provides independent data of the liquid refractive index. The abilities of the device are demonstrated by measuring of biotin molecule binding to a streptavidin monolayer, and by measuring association and dissociation kinetics of immunoglobulin G proteins. Additionally, deposition of PSS / PAH polyelectrolytes is recorded in situ resulting calculation of PSS and PAH monolayer thicknesses separately.
Keywords: label-free sensing; optical surface waves; critical angle refractometry; biotin-streptavidin binding; PSS / PAH multilayer assembly; kinetics of protein binding
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Konopsky, V.N.; Karakouz, T.; Alieva, E.V.; Vicario, C.; Sekatskii, S.K.; Dietler, G. Photonic Crystal Biosensor Based on Optical Surface Waves. Sensors 2013, 13, 2566-2578.
Konopsky VN, Karakouz T, Alieva EV, Vicario C, Sekatskii SK, Dietler G. Photonic Crystal Biosensor Based on Optical Surface Waves. Sensors. 2013; 13(2):2566-2578.
Konopsky, Valery N.; Karakouz, Tanya; Alieva, Elena V.; Vicario, Chiara; Sekatskii, Sergey K.; Dietler, Giovanni. 2013. "Photonic Crystal Biosensor Based on Optical Surface Waves." Sensors 13, no. 2: 2566-2578.