Abstract: A label-free biosensor device based on registration of photonic crystal surface waves is described. Angular interrogation of the optical surface wave resonance is used to detect changes in the thickness of an adsorbed layer, while an additional simultaneous detection of the critical angle of total internal reflection provides independent data of the liquid refractive index. The abilities of the device are demonstrated by measuring of biotin molecule binding to a streptavidin monolayer, and by measuring association and dissociation kinetics of immunoglobulin G proteins. Additionally, deposition of PSS / PAH polyelectrolytes is recorded in situ resulting calculation of PSS and PAH monolayer thicknesses separately.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Konopsky, V.N.; Karakouz, T.; Alieva, E.V.; Vicario, C.; Sekatskii, S.K.; Dietler, G. Photonic Crystal Biosensor Based on Optical Surface Waves. Sensors 2013, 13, 2566-2578.
Konopsky VN, Karakouz T, Alieva EV, Vicario C, Sekatskii SK, Dietler G. Photonic Crystal Biosensor Based on Optical Surface Waves. Sensors. 2013; 13(2):2566-2578.
Konopsky, Valery N.; Karakouz, Tanya; Alieva, Elena V.; Vicario, Chiara; Sekatskii, Sergey K.; Dietler, Giovanni. 2013. "Photonic Crystal Biosensor Based on Optical Surface Waves." Sensors 13, no. 2: 2566-2578.