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Detection of Aeromonas hydrophila in Liquid Media by Volatile Production Similarity Patterns, Using a FF-2A Electronic Nose
Department of Molecular Cell Biology, Institute of DNA Medicine, Research Center for Medical Sciences, The Jikei University School of Medicine, Tokyo 105-8461, Japan
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Research & Development Group, Business Development Department, Analytical & Measuring Instruments Division, Shimadzu Corporation Ltd., Kyoto 604-8442, Japan
Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan
* Author to whom correspondence should be addressed.
Received: 7 December 2012; in revised form: 25 December 2012 / Accepted: 31 December 2012 / Published: 7 January 2013
Abstract: A technique for rapid detection of pathogenic microorganisms is essential for the diagnosis of associated infections and for food safety analysis. Aeromonas hydrophila is one such food contaminant. Several methods for rapid detection of this pathogen have been developed; these include multiplex polymerase chain reaction assays and the colony overlay procedure for peptidases. However, these conventional methods can only be used to detect the microorganisms at high accuracy after symptomatic onset of the disease. Therefore, in the future, simple pre-screening methods may be useful for preventing food poisoning and disease. In this paper, we present a novel system for the rapid detection of the microorganism A. hydrophila in cultured media (in <2 h), with the use of an electronic nose (FF-2A). With this electronic nose, we detected the changes of volatile patterns produced by A. hydrophila after 30 min culture. Our calculations revealed that the increased volatiles were similar to the odours of organic acids and esters. In future, distinctive volatile production patterns of microorganisms identified with the electronic nose may have the potential in microorganism detection.
Keywords: Aeromonas hydrophila; pre-screening; rapid detection; volatile production patterns; electronic nose; FF-2A
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Fujioka, K.; Arakawa, E.; Kita, J.-I.; Aoyama, Y.; Manome, Y.; Ikeda, K.; Yamamoto, K. Detection of Aeromonas hydrophila in Liquid Media by Volatile Production Similarity Patterns, Using a FF-2A Electronic Nose. Sensors 2013, 13, 736-745.
Fujioka K, Arakawa E, Kita J-I, Aoyama Y, Manome Y, Ikeda K, Yamamoto K. Detection of Aeromonas hydrophila in Liquid Media by Volatile Production Similarity Patterns, Using a FF-2A Electronic Nose. Sensors. 2013; 13(1):736-745.
Fujioka, Kouki; Arakawa, Eiji; Kita, Jun-ichi; Aoyama, Yoshihiro; Manome, Yoshinobu; Ikeda, Keiichi; Yamamoto, Kenji. 2013. "Detection of Aeromonas hydrophila in Liquid Media by Volatile Production Similarity Patterns, Using a FF-2A Electronic Nose." Sensors 13, no. 1: 736-745.