Sensors 2013, 13(1), 455-462; doi:10.3390/s130100455
Article

Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response

1 Laboratory of Nanomedicine, Korea Institute of Science and Technology Europe (KIST-Europe), Campus E 7.1, Saarbrücken, Germany 2 Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn-Str. 11, 44227 Dortmund, Germany 3 Department of Life Science, Chonbuk National University, Jeon Ju 561-756, Korea
* Author to whom correspondence should be addressed.
Received: 30 October 2012; in revised form: 19 December 2012 / Accepted: 20 December 2012 / Published: 27 December 2012
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Germany 2012)
PDF Full-text Download PDF Full-Text [325 KB, uploaded 27 December 2012 11:02 CET]
Abstract: Bacteriorhodopsin protein (bR)-based systems are one of the simplest known biological energy converters. The robust chemical, thermal and electrochemical properties of bR have made it an attractive material for photoelectric devices. This study demonstrates the photoelectric response of a dry bR layer deposited on a nitrocellulose membrane with indium tin oxide (ITO) electrodes. Light-induced electrical current as well as potential and impedance changes of dried bR film were recorded as the function of illumination. We have also tested bR in solution and found that the electrical properties are strongly dependent on light intensity changing locally proton concentration and thus pH of the solution. Experimental data support the assumption that bR protein on a positively charged nitrocellulose membrane (PNM) can be used as highly sensitive photo- and pH detector. Here the bR layer facilitates proton translocation and acts as an ultrafast optoelectric signal transducer. It is therefore useful in applications related to bioelectronics, biosensors, bio-optics devices and current carrying junction devices.
Keywords: bacteriorhodopsin (bR); purple membrane (PM); indium tin oxide (ITO); positively charged nitrocellulose membrane (PNM)

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Kim, Y.J.; Neuzil, P.; Nam, C.-H.; Engelhard, M. Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response. Sensors 2013, 13, 455-462.

AMA Style

Kim YJ, Neuzil P, Nam C-H, Engelhard M. Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response. Sensors. 2013; 13(1):455-462.

Chicago/Turabian Style

Kim, Young J.; Neuzil, Pavel; Nam, Chang-Hoon; Engelhard, Martin. 2013. "Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response." Sensors 13, no. 1: 455-462.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert