Sensors 2013, 13(1), 455-462; doi:10.3390/s130100455
Article

Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response

Received: 30 October 2012; in revised form: 19 December 2012 / Accepted: 20 December 2012 / Published: 27 December 2012
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in Germany 2012)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Bacteriorhodopsin protein (bR)-based systems are one of the simplest known biological energy converters. The robust chemical, thermal and electrochemical properties of bR have made it an attractive material for photoelectric devices. This study demonstrates the photoelectric response of a dry bR layer deposited on a nitrocellulose membrane with indium tin oxide (ITO) electrodes. Light-induced electrical current as well as potential and impedance changes of dried bR film were recorded as the function of illumination. We have also tested bR in solution and found that the electrical properties are strongly dependent on light intensity changing locally proton concentration and thus pH of the solution. Experimental data support the assumption that bR protein on a positively charged nitrocellulose membrane (PNM) can be used as highly sensitive photo- and pH detector. Here the bR layer facilitates proton translocation and acts as an ultrafast optoelectric signal transducer. It is therefore useful in applications related to bioelectronics, biosensors, bio-optics devices and current carrying junction devices.
Keywords: bacteriorhodopsin (bR); purple membrane (PM); indium tin oxide (ITO); positively charged nitrocellulose membrane (PNM)
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MDPI and ACS Style

Kim, Y.J.; Neuzil, P.; Nam, C.-H.; Engelhard, M. Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response. Sensors 2013, 13, 455-462.

AMA Style

Kim YJ, Neuzil P, Nam C-H, Engelhard M. Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response. Sensors. 2013; 13(1):455-462.

Chicago/Turabian Style

Kim, Young J.; Neuzil, Pavel; Nam, Chang-Hoon; Engelhard, Martin. 2013. "Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response." Sensors 13, no. 1: 455-462.

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