Sensors 2012, 12(9), 12506-12518; doi:10.3390/s120912506

A SPR Aptasensor for Detection of Avian Influenza Virus H5N1

1email, 1email, 2email, 3email and 1,2,* email
Received: 30 July 2012; in revised form: 27 August 2012 / Accepted: 28 August 2012 / Published: 13 September 2012
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Rapid and specific detection of avian influenza virus (AIV) is urgently needed due to the concerns over the potential outbreaks of highly pathogenic H5N1 influenza in animals and humans. Aptamers are artificial oligonucleic acids that can bind specific target molecules, and show comparable affinity for target viruses and better thermal stability than monoclonal antibodies. The objective of this research was to use a DNA-aptamer as the specific recognition element in a portable Surface Plasmon Resonance (SPR) biosensor for rapid detection of AIV H5N1 in poultry swab samples. A SPR biosensor was fabricated using selected aptamers that were biotinylated and then immobilized on the sensor gold surface coated with streptavidin via streptavidin-biotin binding. The immobilized aptamers captured AIV H5N1 in a sample solution, which caused an increase in the refraction index (RI). After optimizing the streptavidin and aptamer parameters, the results showed that the RI value was linearly related (R2 = 0.99) to the concentration of AIV in the range of 0.128 to 1.28 HAU. Negligible signal ( < 4% of H5N1) was observed from six non-target AIV subtypes. The AIV H5N1 in poultry swab samples with concentrations of 0.128 to 12.8 HAU could be detected using this aptasensor in 1.5 h.
Keywords: aptamer; surface plasmon resonance; biosensor; avian influenza virus
PDF Full-text Download PDF Full-Text [387 KB, uploaded 21 June 2014 04:38 CEST]

Export to BibTeX |

MDPI and ACS Style

Bai, H.; Wang, R.; Hargis, B.; Lu, H.; Li, Y. A SPR Aptasensor for Detection of Avian Influenza Virus H5N1. Sensors 2012, 12, 12506-12518.

AMA Style

Bai H, Wang R, Hargis B, Lu H, Li Y. A SPR Aptasensor for Detection of Avian Influenza Virus H5N1. Sensors. 2012; 12(9):12506-12518.

Chicago/Turabian Style

Bai, Hua; Wang, Ronghui; Hargis, Billy; Lu, Huaguang; Li, Yanbin. 2012. "A SPR Aptasensor for Detection of Avian Influenza Virus H5N1." Sensors 12, no. 9: 12506-12518.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert