Next Article in Journal
A Linear, Millimetre Displacement-to-Frequency Transducer
Next Article in Special Issue
Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays
Previous Article in Journal
Vision-Based Sensor for Early Detection of Periodical Defects in Web Materials
Previous Article in Special Issue
Investigating the Quantitative Structure-Activity Relationships for Antibody Recognition of Two Immunoassays for Polycyclic Aromatic Hydrocarbons by Multiple Regression Methods
Article Menu

Export Article

Open AccessCommunication
Sensors 2012, 12(8), 10810-10819; doi:10.3390/s120810810

Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

1
Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea
2
Bioprocess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea
3
Biotechnology Research Division, National Fisheries Research & Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Korea
4
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea
*
Authors to whom correspondence should be addressed.
Received: 4 July 2012 / Revised: 26 July 2012 / Accepted: 31 July 2012 / Published: 6 August 2012
(This article belongs to the Special Issue Bioassays)
View Full-Text   |   Download PDF [824 KB, uploaded 21 June 2014]   |  

Abstract

Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device. View Full-Text
Keywords: immunosensor; H1N1 influenza; microfluidics; plastic chip immunosensor; H1N1 influenza; microfluidics; plastic chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, K.G.; Lee, T.J.; Jeong, S.W.; Choi, H.W.; Heo, N.S.; Park, J.Y.; Park, T.J.; Lee, S.J. Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza. Sensors 2012, 12, 10810-10819.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top