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Sensors 2012, 12(8), 10810-10819; doi:10.3390/s120810810

Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

4,*  and 1,*
1 Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea 2 Bioprocess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea 3 Biotechnology Research Division, National Fisheries Research & Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Korea 4 Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea
* Authors to whom correspondence should be addressed.
Received: 4 July 2012 / Revised: 26 July 2012 / Accepted: 31 July 2012 / Published: 6 August 2012
(This article belongs to the Special Issue Bioassays)
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Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.
Keywords: immunosensor; H1N1 influenza; microfluidics; plastic chip immunosensor; H1N1 influenza; microfluidics; plastic chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Lee, K.G.; Lee, T.J.; Jeong, S.W.; Choi, H.W.; Heo, N.S.; Park, J.Y.; Park, T.J.; Lee, S.J. Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza. Sensors 2012, 12, 10810-10819.

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