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Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza
Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea
Bioprocess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea
Biotechnology Research Division, National Fisheries Research & Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Korea
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea
* Authors to whom correspondence should be addressed.
Received: 4 July 2012; in revised form: 26 July 2012 / Accepted: 31 July 2012 / Published: 6 August 2012
(This article belongs to the Special Issue Bioassays
Abstract: Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.
Keywords: immunosensor; H1N1 influenza; microfluidics; plastic chip
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MDPI and ACS Style
Lee, K.G.; Lee, T.J.; Jeong, S.W.; Choi, H.W.; Heo, N.S.; Park, J.Y.; Park, T.J.; Lee, S.J. Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza. Sensors 2012, 12, 10810-10819.
Lee KG, Lee TJ, Jeong SW, Choi HW, Heo NS, Park JY, Park TJ, Lee SJ. Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza. Sensors. 2012; 12(8):10810-10819.
Lee, Kyoung G.; Lee, Tae Jae; Jeong, Soon Woo; Choi, Ho Woon; Heo, Nam Su; Park, Jung Youn; Park, Tae Jung; Lee, Seok Jae. 2012. "Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza." Sensors 12, no. 8: 10810-10819.