Sensors 2012, 12(8), 10136-10147; doi:10.3390/s120810136
Article

Synthesis of Bioactive Microcapsules Using a Microfluidic Device

1,2,†email, 1,2,†email, 1email, 3email, 4email, 5email, 1,* email and 2,* email
Received: 8 June 2012; in revised form: 4 July 2012 / Accepted: 18 July 2012 / Published: 26 July 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
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Abstract: Bioactive microcapsules containing Bacillus thuringiensis (BT) spores were generated by a combination of a hydro gel, microfluidic device and chemical polymerization method. As a proof-of-principle, we used BT spores displaying enhanced green fluorescent protein (EGFP) on the spore surface to spatially direct the EGFP-presenting spores within microcapsules. BT spore-encapsulated microdroplets of uniform size and shape are prepared through a flow-focusing method in a microfluidic device and converted into microcapsules through hydrogel polymerization. The size of microdroplets can be controlled by changing both the dispersion and continuous flow rate. Poly(N-isoproplyacrylamide) (PNIPAM), known as a hydrogel material, was employed as a biocompatible material for the encapsulation of BT spores and long-term storage and outstanding stability. Due to these unique properties of PNIPAM, the nutrients from Luria-Bertani complex medium diffused into the microcapsules and the microencapsulated spores germinated into vegetative cells under adequate environmental conditions. These results suggest that there is no limitation of transferring low-molecular-weight-substrates through the PNIPAM structures, and the viability of microencapsulated spores was confirmed by the culture of vegetative cells after the germinations. This microfluidic-based microencapsulation methodology provides a unique way of synthesizing bioactive microcapsules in a one-step process. This microfluidic-based strategy would be potentially suitable to produce microcapsules of various microbial spores for on-site biosensor analysis.
Keywords: microcapsulation; NIPAM; hydrogel; microfluidic device; spore
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kim, B.I.; Jeong, S.W.; Lee, K.G.; Park, T.J.; Park, J.Y.; Song, J.J.; Lee, S.J.; Lee, C.-S. Synthesis of Bioactive Microcapsules Using a Microfluidic Device. Sensors 2012, 12, 10136-10147.

AMA Style

Kim BI, Jeong SW, Lee KG, Park TJ, Park JY, Song JJ, Lee SJ, Lee C-S. Synthesis of Bioactive Microcapsules Using a Microfluidic Device. Sensors. 2012; 12(8):10136-10147.

Chicago/Turabian Style

Kim, Byeong Il; Jeong, Soon Woo; Lee, Kyoung G.; Park, Tae Jung; Park, Jung Youn; Song, Jae Jun; Lee, Seok Jae; Lee, Chang-Soo. 2012. "Synthesis of Bioactive Microcapsules Using a Microfluidic Device." Sensors 12, no. 8: 10136-10147.


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