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Novel Biochip Platform for Nucleic Acid Analysis
Sensors 2012, 12(6), 8319-8337; doi:10.3390/s120608319

Diagnostic Devices for Isothermal Nucleic Acid Amplification

1 Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan 2 Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan 3 Center for Emerging Material and Advanced Devices, National Taiwan University, Taipei 10617, Taiwan
* Author to whom correspondence should be addressed.
Received: 2 May 2012 / Revised: 29 May 2012 / Accepted: 7 June 2012 / Published: 14 June 2012
(This article belongs to the Special Issue Biomarkers and Nanosensors: New Approaches for Biology and Medicine)
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Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.
Keywords: isothermal nucleic acid amplification; NASBA; SDA; RCA; LAMP; HDA isothermal nucleic acid amplification; NASBA; SDA; RCA; LAMP; HDA
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chang, C.-C.; Chen, C.-C.; Wei, S.-C.; Lu, H.-H.; Liang, Y.-H.; Lin, C.-W. Diagnostic Devices for Isothermal Nucleic Acid Amplification. Sensors 2012, 12, 8319-8337.

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