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Sensors 2012, 12(6), 8319-8337; doi:10.3390/s120608319
Review

Diagnostic Devices for Isothermal Nucleic Acid Amplification

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Received: 2 May 2012; in revised form: 29 May 2012 / Accepted: 7 June 2012 / Published: 14 June 2012
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Abstract: Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.
Keywords: isothermal nucleic acid amplification; NASBA; SDA; RCA; LAMP; HDA isothermal nucleic acid amplification; NASBA; SDA; RCA; LAMP; HDA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chang, C.-C.; Chen, C.-C.; Wei, S.-C.; Lu, H.-H.; Liang, Y.-H.; Lin, C.-W. Diagnostic Devices for Isothermal Nucleic Acid Amplification. Sensors 2012, 12, 8319-8337.

AMA Style

Chang C-C, Chen C-C, Wei S-C, Lu H-H, Liang Y-H, Lin C-W. Diagnostic Devices for Isothermal Nucleic Acid Amplification. Sensors. 2012; 12(6):8319-8337.

Chicago/Turabian Style

Chang, Chia-Chen; Chen, Chien-Cheng; Wei, Shih-Chung; Lu, Hui-Hsin; Liang, Yang-Hung; Lin, Chii-Wann. 2012. "Diagnostic Devices for Isothermal Nucleic Acid Amplification." Sensors 12, no. 6: 8319-8337.


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