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• Pernagallo, S
• Ventimiglia, G
• Cavalluzzo, C
• Alessi, E
• Ilyine, H
• Bradley, M
• Diaz-Mochon, J
• [+] on Google Scholar
• Pernagallo, S
• Ventimiglia, G
• Cavalluzzo, C
• Alessi, E
• Ilyine, H
• Bradley, M
• Diaz-Mochon, J
• [+] on PubMed
• Pernagallo, S
• Ventimiglia, G
• Cavalluzzo, C
• Alessi, E
• Ilyine, H
• Bradley, M
• Diaz-Mochon, J

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Sensors 2012, 12(6), 8100-8111; doi:10.3390/s120608100

Article

Novel Biochip Platform for Nucleic Acid Analysis

Salvatore Pernagallo ^1,† , Giorgio Ventimiglia ^2,†,* , Claudia Cavalluzzo ¹ , Enrico Alessi ² , Hugh Ilyine ¹ , Mark Bradley ³  and Juan J. Diaz-Mochon ^1,*

¹ DestiNA Genomics Ltd., West Mains Road, Edinburgh EH9 3JJ, UK ² STMicroelectronics, ANALOG, MEMS and SENSORS Group, Healthcare BDU, Stradale Primosole 50, Catania 95121, Italy ³ School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 25 April 2012; in revised form: 21 May 2012 / Accepted: 31 May 2012 / Published: 11 June 2012

(This article belongs to the Special Issue Biomarkers and Nanosensors: New Approaches for Biology and Medicine)

Download PDF Full-Text [938 KB, uploaded 11 June 2012 10:57 CEST]

Abstract: This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top “footprint” requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

Keywords: Lab-on-Chip (LoC); microarray; nucleic acid; dynamic chemistry; polymerase chain reaction (PCR); peptide nucleic acid (PNA); microfluidic; single nucleotide polymorphism (SNP); microRNA-122 (miRNA122); mengo virus (MGV); nucleic acid test (NAT); point-of-care (POC)

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