Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2012, 12(5), 6176-6185; doi:10.3390/s120506176
Article

luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?

1,†
, 1,†
, 1,2
, 1
, 1
, 1
, 1,* , 1
 and 1,*
Received: 19 March 2012; in revised form: 12 April 2012 / Accepted: 20 April 2012 / Published: 10 May 2012
View Full-Text   |   Download PDF [436 KB, uploaded 21 June 2014]   |   Browse Figures
Abstract: AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation.
Keywords: quorum sensing; AI-2; luxS; sahH quorum sensing; AI-2; luxS; sahH
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, Q.; He, Z.; Hu, Y.; Jiang, Y.; Ma, R.; Tang, Z.; Liang, J.; Liu, Z.; Huang, Z. luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Sensors 2012, 12, 6176-6185.

AMA Style

Wang Q, He Z, Hu Y, Jiang Y, Ma R, Tang Z, Liang J, Liu Z, Huang Z. luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder? Sensors. 2012; 12(5):6176-6185.

Chicago/Turabian Style

Wang, Qian; He, Zhiyan; Hu, Yuejian; Jiang, Yuntao; Ma, Rui; Tang, Zisheng; Liang, Jingping; Liu, Zheng; Huang, Zhengwei. 2012. "luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?" Sensors 12, no. 5: 6176-6185.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert