Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2012, 12(5), 5596-5607; doi:10.3390/s120505596
Article

Glycopeptide-Based Antibody Detection in Multiple Sclerosis by Surface Plasmon Resonance

1,2
, 1,3
, 4
, 1,2
, 1,5
, 1,2,4
 and 1,3,*
Received: 1 March 2012; in revised form: 13 April 2012 / Accepted: 27 April 2012 / Published: 2 May 2012
View Full-Text   |   Download PDF [334 KB, uploaded 21 June 2014]
Abstract: In multiple sclerosis (MS) the gold standard for the diagnosis and prognosis is, up to now, the use of magnetic resonance imaging markers. No alternative simpler assays proven of use, except for cerebrospinal fluid analysis, have been provided in MS diagnosis. Therefore, there is a need to develop non-invasive, sensitive, simple new techniques for the clinical routine. Herein we present the evaluation of the feasibility of a glycopeptide-based biosensor to detect MS specific antibodies in sera using the surface plasmon resonance technology. The previously described glycopeptide antigen CSF114(Glc) has been immobilized on a gold sensor chip and the method has been optimized for real-time specific autoantibody detection directly in sera. A population of 60 healthy blood donors and 61 multiple sclerosis patients has been screened. The receiver operating characteristic (ROC)-based analysis has established the optimal diagnostic cut-off value for the method obtaining a sensitivity of 36% and a specificity of 95%. Sample sera have been also screened with a previously validated ELISA.
Keywords: multiple sclerosis; surface plasmon resonance; serodiagnosis; biacore; immunoassay; glycopeptide CSF114(Glc) multiple sclerosis; surface plasmon resonance; serodiagnosis; biacore; immunoassay; glycopeptide CSF114(Glc)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Real-Fernández, F.; Passalacqua, I.; Peroni, E.; Chelli, M.; Lolli, F.; Papini, A.M.; Rovero, P. Glycopeptide-Based Antibody Detection in Multiple Sclerosis by Surface Plasmon Resonance. Sensors 2012, 12, 5596-5607.

AMA Style

Real-Fernández F, Passalacqua I, Peroni E, Chelli M, Lolli F, Papini AM, Rovero P. Glycopeptide-Based Antibody Detection in Multiple Sclerosis by Surface Plasmon Resonance. Sensors. 2012; 12(5):5596-5607.

Chicago/Turabian Style

Real-Fernández, Feliciana; Passalacqua, Irene; Peroni, Elisa; Chelli, Mario; Lolli, Francesco; Papini, Anna Maria; Rovero, Paolo. 2012. "Glycopeptide-Based Antibody Detection in Multiple Sclerosis by Surface Plasmon Resonance." Sensors 12, no. 5: 5596-5607.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert