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Sensors 2012, 12(4), 4897-4917; doi:10.3390/s120404897

Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination

1,* , 1
1 Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania 2 Institute of Biochemistry, Vilnius University, Mokslininku 12, LT-08662 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 6 March 2012 / Revised: 2 April 2012 / Accepted: 4 April 2012 / Published: 16 April 2012
(This article belongs to the Section Biosensors)
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In this paper the operation of an amperometric biosensor producing a chemically amplified signal is modelled numerically. The chemical amplification is achieved by using synergistic substrates. The model is based on non-stationary reaction-diffusion equations. The model involves three layers (compartments): a layer of enzyme solution entrapped on the electrode surface, a dialysis membrane covering the enzyme layer and an outer diffusion layer which is modelled by the Nernst approach. The equation system is solved numerically by using the finite difference technique. The biosensor response and sensitivity are investigated by altering the model parameters influencing the enzyme kinetics as well as the mass transport by diffusion. The biosensor action was analyzed with a special emphasis to the effect of the chemical amplification. The simulation results qualitatively explain and confirm the experimentally observed effect of the synergistic substrates conversion on the biosensor response.
Keywords: modelling; simulation; reaction-diffusion; biosensor; synergistic substrates modelling; simulation; reaction-diffusion; biosensor; synergistic substrates
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Simelevicius, D.; Baronas, R.; Kulys, J. Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination. Sensors 2012, 12, 4897-4917.

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