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Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination
Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania
Institute of Biochemistry, Vilnius University, Mokslininku 12, LT-08662 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 6 March 2012; in revised form: 2 April 2012 / Accepted: 4 April 2012 / Published: 16 April 2012
Abstract: In this paper the operation of an amperometric biosensor producing a chemically amplified signal is modelled numerically. The chemical amplification is achieved by using synergistic substrates. The model is based on non-stationary reaction-diffusion equations. The model involves three layers (compartments): a layer of enzyme solution entrapped on the electrode surface, a dialysis membrane covering the enzyme layer and an outer diffusion layer which is modelled by the Nernst approach. The equation system is solved numerically by using the finite difference technique. The biosensor response and sensitivity are investigated by altering the model parameters influencing the enzyme kinetics as well as the mass transport by diffusion. The biosensor action was analyzed with a special emphasis to the effect of the chemical amplification. The simulation results qualitatively explain and confirm the experimentally observed effect of the synergistic substrates conversion on the biosensor response.
Keywords: modelling; simulation; reaction-diffusion; biosensor; synergistic substrates
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MDPI and ACS Style
Simelevicius, D.; Baronas, R.; Kulys, J. Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination. Sensors 2012, 12, 4897-4917.
Simelevicius D, Baronas R, Kulys J. Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination. Sensors. 2012; 12(4):4897-4917.
Simelevicius, Dainius; Baronas, Romas; Kulys, Juozas. 2012. "Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination." Sensors 12, no. 4: 4897-4917.