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A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249
Microbial Culture Collection, National Centre for Cell Science, Pune, Maharashtra 411007, India
National Institute of Oceanography, Dr. Salim Ali Road, P.O. Box 1913, Kochi, Kerala 682018, India
* Author to whom correspondence should be addressed.
Received: 21 February 2012; in revised form: 17 March 2012 / Accepted: 20 March 2012 / Published: 26 March 2012
Abstract: We have investigated the quorum sensing control in Aeromonas veronii MTCC 3249, originally isolated as A. culicicola from the midgut of Culex quinquefasciatus. Based on biosensor assays, the bacterium showed constant production of multiple acyl-homoserine lactones (AHLs) with increasing cell-density. The luxRI gene homologs, acuR (A. culicicola transcriptional Regulator) and acuI (A. culicicola autoInducer) were successfully amplified by inverse-PCR. Sequence analysis indicated acuRI were divergent from all known quorum sensing gene homologs in Aeromonas. Two localized regions in the C-terminal autoinducer binding domain of acuR showed indels suggesting variations in autoinducer specificity. Further, only a single copy of the quorum sensing genes was detected, suggesting a tight regulation of mechanisms under its control. Chromatography and further chemical analysis identified two AHLs in the culture supernatant: 6-carboxy-HHL (homoadipyl homoserine lactone), a novel AHL, and N-tetradecanoylhomoserine lactone. The existence of a potentially variant quorum sensing system might therefore, reflect in some way the ecological strategies adopted by this bacterium in the mosquito midgut.
Keywords: Aeromonas; luxRI homolog; acuR; acuI; quorum sensing; AHL; mosquito midgut; 6-carboxy-HHL
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Jangid, K.; Parameswaran, P.S.; Shouche, Y.S. A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249. Sensors 2012, 12, 3814-3830.
Jangid K, Parameswaran PS, Shouche YS. A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249. Sensors. 2012; 12(4):3814-3830.
Jangid, Kamlesh; Parameswaran, Perunninakulath S.; Shouche, Yogesh S. 2012. "A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249." Sensors 12, no. 4: 3814-3830.