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Sensors 2012, 12(3), 2969-2982; doi:10.3390/s120302969
Article

An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media

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Received: 17 January 2012; in revised form: 23 February 2012 / Accepted: 23 February 2012 / Published: 2 March 2012
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Abstract: The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water ‘‘dip-in’’ fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a “turn-on” pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.
Keywords: sensory materials; chemosensor; fluorogenic sensor; biomolecules; sensing biomolecules sensory materials; chemosensor; fluorogenic sensor; biomolecules; sensing biomolecules
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Vallejos, S.; Estévez, P.; Ibeas, S.; García, F.C.; Serna, F.; García, J.M. An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media. Sensors 2012, 12, 2969-2982.

AMA Style

Vallejos S, Estévez P, Ibeas S, García FC, Serna F, García JM. An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media. Sensors. 2012; 12(3):2969-2982.

Chicago/Turabian Style

Vallejos, Saúl; Estévez, Pedro; Ibeas, Saturnino; García, Félix C.; Serna, Felipe; García, José M. 2012. "An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media." Sensors 12, no. 3: 2969-2982.


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