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Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis
Department of Biomechatronic Engineering, National Chiayi University, No. 300, University Rd., East Dist., Chiayi 600, Taiwan
* Author to whom correspondence should be addressed.
Received: 19 January 2012; in revised form: 13 February 2012 / Accepted: 21 February 2012 / Published: 23 February 2012
Abstract: This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.
Keywords: electroosmotic flow; cell lysis; dielectrophoresis force; cell manipulation
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MDPI and ACS Style
Young, C.-W.; Hsieh, J.-L.; Ay, C. Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis. Sensors 2012, 12, 2400-2413.
Young C-W, Hsieh J-L, Ay C. Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis. Sensors. 2012; 12(3):2400-2413.
Young, Chao-Wang; Hsieh, Jia-Ling; Ay, Chyung. 2012. "Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis." Sensors 12, no. 3: 2400-2413.