Sensors 2012, 12(2), 1544-1571; doi:10.3390/s120201544
Review

Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History

1,* email, 2email, 2email, 2email, 3email and 2email
Received: 29 December 2011; in revised form: 24 January 2012 / Accepted: 3 February 2012 / Published: 6 February 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution.
Keywords: bioluminescence; bioreporter; biosensors; Pseudomonas fluorescens HK44; lux genes
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MDPI and ACS Style

Trögl, J.; Chauhan, A.; Ripp, S.; Layton, A.C.; Kuncová, G.; Sayler, G.S. Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History. Sensors 2012, 12, 1544-1571.

AMA Style

Trögl J, Chauhan A, Ripp S, Layton AC, Kuncová G, Sayler GS. Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History. Sensors. 2012; 12(2):1544-1571.

Chicago/Turabian Style

Trögl, Josef; Chauhan, Archana; Ripp, Steven; Layton, Alice C.; Kuncová, Gabriela; Sayler, Gary S. 2012. "Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History." Sensors 12, no. 2: 1544-1571.

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