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Multi-Sensor Arrays for Online Monitoring of Cell Dynamics in in vitro Studies with Choroid Plexus Epithelial Cells
Department of Anatomy and Cell Biology, Saarland University, University Hospital, Bldg. 61, 66421 Homburg, Saar, Germany
Department of Human Histology and Pathology, Faculty for Health Sciences, University Rey Juan Carlos I, Av. de Atenas s/n, E-28922 Alcorcón, Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 1 December 2011; in revised form: 13 January 2012 / Accepted: 17 January 2012 / Published: 1 February 2012
Abstract: Sensors and multi-sensor arrays are the basis of new technologies for the non-label monitoring of cell activity. In this paper we show that choroid plexus cells can be cultured on silicon chips and that sensors register in real time changes in their activity, constituting an interesting experimental paradigm for cell biology and medical research. To validate the signals recorded (metabolism = peri-cellular acidification, oxygen consumption = respiration; impedance = adhesion, cell shape and motility) we performed experiments with compounds that act in a well-known way on cells, influencing these parameters. Our in vitro model demonstrates the advantages of multi-sensor arrays in assessment and experimental characterization of dynamic cellular events—in this case in choroid plexus functions, however with applicability to other cell types as well.
Keywords: sensor arrays; peri-cellular acidosis; cell respiration; cell adhesion; impedance; cell cultures; electron microscopy; choroid plexus; chick embryo
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MDPI and ACS Style
Mestres-Ventura, P.; Morguet, A.; Gómez de las Heras, S.G. Multi-Sensor Arrays for Online Monitoring of Cell Dynamics in in vitro Studies with Choroid Plexus Epithelial Cells. Sensors 2012, 12, 1383-1397.
Mestres-Ventura P, Morguet A, Gómez de las Heras SG. Multi-Sensor Arrays for Online Monitoring of Cell Dynamics in in vitro Studies with Choroid Plexus Epithelial Cells. Sensors. 2012; 12(2):1383-1397.
Mestres-Ventura, Pedro; Morguet, Andrea; Gómez de las Heras, Soledad García. 2012. "Multi-Sensor Arrays for Online Monitoring of Cell Dynamics in in vitro Studies with Choroid Plexus Epithelial Cells." Sensors 12, no. 2: 1383-1397.