Sensors 2012, 12(11), 15078-15087; doi:10.3390/s121115078
Article

Simple, Fast and Selective Detection of Adenosine Triphosphate at Physiological pH Using Unmodified Gold Nanoparticles as Colorimetric Probes and Metal Ions as Cross-Linkers

Dehua Deng 1 email, Ning Xia 1,2,* email, Sujuan Li 1 email, Chunying Xu 1 email, Ting Sun 1 email, Huan Pang 1,* email and Lin Liu 1 email
1 College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China 2 College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China
* Authors to whom correspondence should be addressed.
Received: 8 October 2012; in revised form: 24 October 2012 / Accepted: 2 November 2012 / Published: 6 November 2012
(This article belongs to the Special Issue Multimetallic Nanoparticles for Chemical/Biological Sensing Applications)
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Abstract: We report a simple, fast and selective colorimetric assay of adenosine triphosphate (ATP) using unmodified gold nanoparticles (AuNPs) as probes and metal ions as cross-linkers. ATP can be assembled onto the surface of AuNPs through interaction between the electron-rich nitrogen atoms and the electron-deficient surface of AuNPs. Accordingly, Cu2+ ions induce a change in the color and UV/Vis absorbance of AuNPs by coordinating to the triphosphate groups and a ring nitrogen of ATP. A detection limit of 50 nM was achieved, which is comparable to or lower than that achievable by the currently used electrochemical, spectroscopic or chromatographic methods. The theoretical simplicity and high selectivity reported herein demonstrated that AuNPs-based colorimetric assay could be applied in a wide variety of fields by rationally designing the surface chemistry of AuNPs. In addition, our results indicate that ATP-modified AuNPs are less stable in Cu2+, Cd2+ or Zn2+-containing solutions due to the formation of the corresponding dimeric metal-ATP complexes.
Keywords: gold nanoparticles; adenosine triphosphate; colorimetric assay; metal ions

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MDPI and ACS Style

Deng, D.; Xia, N.; Li, S.; Xu, C.; Sun, T.; Pang, H.; Liu, L. Simple, Fast and Selective Detection of Adenosine Triphosphate at Physiological pH Using Unmodified Gold Nanoparticles as Colorimetric Probes and Metal Ions as Cross-Linkers. Sensors 2012, 12, 15078-15087.

AMA Style

Deng D, Xia N, Li S, Xu C, Sun T, Pang H, Liu L. Simple, Fast and Selective Detection of Adenosine Triphosphate at Physiological pH Using Unmodified Gold Nanoparticles as Colorimetric Probes and Metal Ions as Cross-Linkers. Sensors. 2012; 12(11):15078-15087.

Chicago/Turabian Style

Deng, Dehua; Xia, Ning; Li, Sujuan; Xu, Chunying; Sun, Ting; Pang, Huan; Liu, Lin. 2012. "Simple, Fast and Selective Detection of Adenosine Triphosphate at Physiological pH Using Unmodified Gold Nanoparticles as Colorimetric Probes and Metal Ions as Cross-Linkers." Sensors 12, no. 11: 15078-15087.

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