Sensors 2012, 12(10), 13471-13479; doi:10.3390/s121013471
A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles
1
Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China
2
Department of Biomedical engineering, Chongqing University, 174 Shazhengjie, Shapingba District, Chongqing 400044, China
*
Author to whom correspondence should be addressed.
Received: 8 August 2012 / Revised: 7 September 2012 / Accepted: 20 September 2012 / Published: 8 October 2012
(This article belongs to the Special Issue Bioassays)
Abstract
CD105 is a well-known tumor metastasis marker and useful for early monitoring of metastasis and cancer relapse. It is important to generate rapid, reliable and precise analytical information regarding CD105 levels. To establish a simple, selective and sensitive detection method, we prepared an immunosensor with novel bioconjugates based on Pt nanoparticles, thionin acetate and antibodies. The proposed immunosensor displayed a broader linear response to CD105, with a working range of 1.3 to 200.0 ng/mL and a detection limit of 0.9 ng/mL under optimal conditions. Moreover, the studied immunosensor exhibited high sensitivity, fast analysis and adequate stability. The proposed methodology could readily be extended to other clinical- or environment-related biospecies.Keywords:
CD105; immunosensor; nanomaterial
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Zeng, S.; Wang, S.; Wang, L.; Yang, L.; Chen, Z.; Liang, Z. A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles. Sensors 2012, 12, 13471-13479.