A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles

doi:10.3390/s121013471

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Sensors 2012, 12(10), 13471-13479; doi:10.3390/s121013471

Article

A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles

Suyun Zeng¹, Sujun Wang², Li Wang¹, Lihua Yang¹, Zhenbo Chen¹ and Zhiqing Liang^1,*

¹ Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China ² Department of Biomedical engineering, Chongqing University, 174 Shazhengjie, Shapingba District, Chongqing 400044, China

* Author to whom correspondence should be addressed.

Received: 8 August 2012; in revised form: 7 September 2012 / Accepted: 20 September 2012 / Published: 8 October 2012

(This article belongs to the Special Issue Bioassays)

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Abstract: CD105 is a well-known tumor metastasis marker and useful for early monitoring of metastasis and cancer relapse. It is important to generate rapid, reliable and precise analytical information regarding CD105 levels. To establish a simple, selective and sensitive detection method, we prepared an immunosensor with novel bioconjugates based on Pt nanoparticles, thionin acetate and antibodies. The proposed immunosensor displayed a broader linear response to CD105, with a working range of 1.3 to 200.0 ng/mL and a detection limit of 0.9 ng/mL under optimal conditions. Moreover, the studied immunosensor exhibited high sensitivity, fast analysis and adequate stability. The proposed methodology could readily be extended to other clinical- or environment-related biospecies.

Keywords: CD105; immunosensor; nanomaterial

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MDPI and ACS Style

Zeng, S.; Wang, S.; Wang, L.; Yang, L.; Chen, Z.; Liang, Z. A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles. Sensors 2012, 12, 13471-13479.

AMA Style

Zeng S, Wang S, Wang L, Yang L, Chen Z, Liang Z. A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles. Sensors. 2012; 12(10):13471-13479.

Chicago/Turabian Style

Zeng, Suyun; Wang, Sujun; Wang, Li; Yang, Lihua; Chen, Zhenbo; Liang, Zhiqing. 2012. "A Novel CD105 Determination System Based on an Ultrasensitive Bioelectrochemical Strategy with Pt Nanoparticles." Sensors 12, no. 10: 13471-13479.

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