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A Guided Mode Resonance Aptasensor for Thrombin Detection
Department of Optics and Photonics, National Central University, Jhongli 32001, Taiwan
Department of Chemical and Materials Engineering, National Central University, Jhongli 32001, Taiwan
Optical Sciences Center, National Central University, Jhongli 32001, Taiwan
Center for Dynamical Biomarkers and Translational Medicine, National Central University, Jhongli 32001, Taiwan
* Authors to whom correspondence should be addressed.
Received: 21 July 2011; in revised form: 27 August 2011 / Accepted: 15 September 2011 / Published: 19 September 2011
Abstract: Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (Ka) measured is in the range of 106 M−1. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.
Keywords: aptamer; optical biosensor; guided mode resonance; thrombin; affinity constant
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Lin, S.-F.; Ding, T.-J.; Liu, J.-T.; Lee, C.-C.; Yang, T.-H.; Chen, W.-Y.; Chang, J.-Y. A Guided Mode Resonance Aptasensor for Thrombin Detection. Sensors 2011, 11, 8953-8965.
Lin S-F, Ding T-J, Liu J-T, Lee C-C, Yang T-H, Chen W-Y, Chang J-Y. A Guided Mode Resonance Aptasensor for Thrombin Detection. Sensors. 2011; 11(9):8953-8965.
Lin, Sheng-Fu; Ding, Ting-Jou; Liu, Jen-Tsai; Lee, Chien-Chieh; Yang, Tsung-Hsun; Chen, Wen-Yih; Chang, Jenq-Yang. 2011. "A Guided Mode Resonance Aptasensor for Thrombin Detection." Sensors 11, no. 9: 8953-8965.