Sensors 2011, 11(8), 7879-7891; doi:10.3390/s110807879
Article

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

Kim E. Sapsford 1,* email, Samantha Spindel 1,2 email, Travis Jennings 3 email, Guoliang Tao 3 email, Robert C. Triulzi 3 email, W. Russ Algar 4,5 email and Igor L. Medintz 4,* email
1 Division of Biology, Office of Science and Engineering Laboratories, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA 2 Fischell Department of Bioengineering, University of Maryland, College Park, MD 20993, USA 3 eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA 4 Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, 4555 Overlook Avenue, S.W., Washington, DC 20375, USA 5 College of Science, George Mason University, 4400 University Dr., Fairfax, VA 22030, USA
* Authors to whom correspondence should be addressed.
Received: 25 April 2011; in revised form: 29 July 2011 / Accepted: 1 August 2011 / Published: 11 August 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract: The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took
Keywords: quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry

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Cite This Article

MDPI and ACS Style

Sapsford, K.E.; Spindel, S.; Jennings, T.; Tao, G.; Triulzi, R.C.; Algar, W.R.; Medintz, I.L. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors 2011, 11, 7879-7891.

AMA Style

Sapsford KE, Spindel S, Jennings T, Tao G, Triulzi RC, Algar WR, Medintz IL. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors. 2011; 11(8):7879-7891.

Chicago/Turabian Style

Sapsford, Kim E.; Spindel, Samantha; Jennings, Travis; Tao, Guoliang; Triulzi, Robert C.; Algar, W. Russ; Medintz, Igor L. 2011. "Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats." Sensors 11, no. 8: 7879-7891.

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