Next Article in Journal
A Low-Cost CMOS-MEMS Piezoresistive Accelerometer with Large Proof Mass
Next Article in Special Issue
A Fusion-Spliced Near-Field Optical Fiber Probe Using Photonic Crystal Fiber for Nanoscale Thermometry Based on Fluorescence-Lifetime Measurement of Quantum Dots
Previous Article in Journal
LuxCDABE—Transformed Constitutively Bioluminescent Escherichia coli for Toxicity Screening: Comparison with Naturally Luminous Vibrio fischeri
Previous Article in Special Issue
FRET-Based Quantum Dot Immunoassay for Rapid and Sensitive Detection of Aspergillus amstelodami
Sensors 2011, 11(8), 7879-7891; doi:10.3390/s110807879
Article

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

1,* , 1,2
,
3
,
3
,
3
,
4,5
 and
4,*
Received: 25 April 2011 / Revised: 29 July 2011 / Accepted: 1 August 2011 / Published: 11 August 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [720 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took
Keywords: quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Sapsford, K.E.; Spindel, S.; Jennings, T.; Tao, G.; Triulzi, R.C.; Algar, W.R.; Medintz, I.L. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors 2011, 11, 7879-7891.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert