Next Article in Journal
A Low-Cost CMOS-MEMS Piezoresistive Accelerometer with Large Proof Mass
Next Article in Special Issue
A Fusion-Spliced Near-Field Optical Fiber Probe Using Photonic Crystal Fiber for Nanoscale Thermometry Based on Fluorescence-Lifetime Measurement of Quantum Dots
Previous Article in Journal
LuxCDABE—Transformed Constitutively Bioluminescent Escherichia coli for Toxicity Screening: Comparison with Naturally Luminous Vibrio fischeri
Previous Article in Special Issue
FRET-Based Quantum Dot Immunoassay for Rapid and Sensitive Detection of Aspergillus amstelodami
Sensors 2011, 11(8), 7879-7891; doi:10.3390/s110807879
Article

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

1,* , 1,2
,
3
,
3
,
3
,
4,5
 and
4,*
1 Division of Biology, Office of Science and Engineering Laboratories, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA 2 Fischell Department of Bioengineering, University of Maryland, College Park, MD 20993, USA 3 eBioscience, Inc., 10255 Science Center Drive, San Diego, CA 92121, USA 4 Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, 4555 Overlook Avenue, S.W., Washington, DC 20375, USA 5 College of Science, George Mason University, 4400 University Dr., Fairfax, VA 22030, USA
* Authors to whom correspondence should be addressed.
Received: 25 April 2011 / Revised: 29 July 2011 / Accepted: 1 August 2011 / Published: 11 August 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [720 KB, uploaded 21 June 2014]   |  

Abstract

The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took
Keywords: quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Sapsford, K.E.; Spindel, S.; Jennings, T.; Tao, G.; Triulzi, R.C.; Algar, W.R.; Medintz, I.L. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors 2011, 11, 7879-7891.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert