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Sensors 2011, 11(8), 7879-7891; doi:10.3390/s110807879
Article

Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

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Received: 25 April 2011; in revised form: 29 July 2011 / Accepted: 1 August 2011 / Published: 11 August 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract: The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took
Keywords: quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry quantum dot (QD); nanocrystal (NC); semiconductor; bioconjugation; sensor; multiplex; immunoassay; sulfhydryl chemistry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sapsford, K.E.; Spindel, S.; Jennings, T.; Tao, G.; Triulzi, R.C.; Algar, W.R.; Medintz, I.L. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors 2011, 11, 7879-7891.

AMA Style

Sapsford KE, Spindel S, Jennings T, Tao G, Triulzi RC, Algar WR, Medintz IL. Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats. Sensors. 2011; 11(8):7879-7891.

Chicago/Turabian Style

Sapsford, Kim E.; Spindel, Samantha; Jennings, Travis; Tao, Guoliang; Triulzi, Robert C.; Algar, W. Russ; Medintz, Igor L. 2011. "Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats." Sensors 11, no. 8: 7879-7891.


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