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Sensors 2011, 11(4), 3791-3802; doi:10.3390/s110403791
Article

An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System

1,* , 1
,
2
 and
1
1 Centro de Investigación y de Estudios Avanzados del Intituto Politecnico Nacional, Av. Instituto Politécnico Nacional 2508, Mexico 2 Université de Perpignan Via Domitia, IMAGES EA4218, Centre de Phytopharmacie, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France
* Author to whom correspondence should be addressed.
Received: 12 February 2011 / Revised: 22 March 2011 / Accepted: 24 March 2011 / Published: 28 March 2011
(This article belongs to the Special Issue Bioinspired Sensor Systems)
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Abstract

An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system (MCFA) was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon (CPO), chlorfenvinfos (CFV) and azinphos methyl-oxon (AZMO). The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView® to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved.
Keywords: biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Alonso, G.A.; Dominguez, R.B.; Marty, J.-L.; Muñoz, R. An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System. Sensors 2011, 11, 3791-3802.

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