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Sensors 2011, 11(4), 3791-3802; doi:10.3390/s110403791
Article

An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System

1,* , 1
,
2
 and
1
1 Centro de Investigación y de Estudios Avanzados del Intituto Politecnico Nacional, Av. Instituto Politécnico Nacional 2508, Mexico 2 Université de Perpignan Via Domitia, IMAGES EA4218, Centre de Phytopharmacie, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France
* Author to whom correspondence should be addressed.
Received: 12 February 2011 / Revised: 22 March 2011 / Accepted: 24 March 2011 / Published: 28 March 2011
(This article belongs to the Special Issue Bioinspired Sensor Systems)
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Abstract

An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system (MCFA) was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon (CPO), chlorfenvinfos (CFV) and azinphos methyl-oxon (AZMO). The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView® to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved.
Keywords: biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Alonso, G.A.; Dominguez, R.B.; Marty, J.-L.; Muñoz, R. An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System. Sensors 2011, 11, 3791-3802.

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