• Submit to Sensors Login Register
• MDPI
• Journals A-Z
• For Authors
• For Editors
• About
• Open Access Policy

• Abstract
• Full-Text PDF [666 KB]
• Full-Text XML
• Article Versions Notes

• Article Statistics
• PubMed/Medline
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Kraziński, B
• Radecki, J
• Radecka, H
• [+] on Google Scholar
• Kraziński, B
• Radecki, J
• Radecka, H
• [+] on PubMed
• Kraziński, B
• Radecki, J
• Radecka, H

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Sensors 2011, 11(4), 4030-4042; doi:10.3390/s110404030

Article

Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β Peptide

Bartłomiej Emil Kraziński, Jerzy Radecki and Hanna Radecka*

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Street, 10-747 Olsztyn, Poland

* Author to whom correspondence should be addressed.

Received: 16 February 2011; in revised form: 16 March 2011 / Accepted: 28 March 2011 / Published: 6 April 2011

(This article belongs to the Special Issue Bioinspired Sensor Systems)

Download PDF Full-Text [666 KB, uploaded 6 April 2011 14:53 CEST]

Abstract: The main objective of the presented study was the development of a simple analytical tool for exploring the influence of naturally occurring compounds on the aggregation of amyloid-β peptide (Aβ₄₀) in order to find potential anti-neurodegenerative drugs. The gold discs used for surface plasmon resonance (SPR) measurements were modified with thioaliphatic acid. The surface functionalized with carboxylic groups was used for covalent attaching of Aβ₄₀ probe by creation of amide bonds in the presence of EDC/NHS. The modified SPR gold discs were used for exploring the Aβ₄₀ aggregation process in the presence of selected alkaloids: arecoline hydrobromide, pseudopelletierine hydrochloride, trigonelline hydrochloride and α-lobeline hydrochloride. The obtained results were discussed with other parameters which govern the phenomenon studied such as lipophilicity/ hydrophilicy and Aβ₄₀-alkaloid association constants.

Keywords: amyloid-β peptide; surface plasmon resonance; alkaloids; thioaliphatic acid monolayer

Article Statistics

Cite This Article