Sensors 2011, 11(4), 4030-4042; doi:10.3390/s110404030
Article

Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β Peptide

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Street, 10-747 Olsztyn, Poland
* Author to whom correspondence should be addressed.
Received: 16 February 2011; in revised form: 16 March 2011 / Accepted: 28 March 2011 / Published: 6 April 2011
(This article belongs to the Special Issue Bioinspired Sensor Systems)
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Abstract: The main objective of the presented study was the development of a simple analytical tool for exploring the influence of naturally occurring compounds on the aggregation of amyloid-β peptide (Aβ40) in order to find potential anti-neurodegenerative drugs. The gold discs used for surface plasmon resonance (SPR) measurements were modified with thioaliphatic acid. The surface functionalized with carboxylic groups was used for covalent attaching of Aβ40 probe by creation of amide bonds in the presence of EDC/NHS. The modified SPR gold discs were used for exploring the Aβ40 aggregation process in the presence of selected alkaloids: arecoline hydrobromide, pseudopelletierine hydrochloride, trigonelline hydrochloride and α-lobeline hydrochloride. The obtained results were discussed with other parameters which govern the phenomenon studied such as lipophilicity/ hydrophilicy and Aβ40-alkaloid association constants.
Keywords: amyloid-β peptide; surface plasmon resonance; alkaloids; thioaliphatic acid monolayer

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MDPI and ACS Style

Kraziński, B.E.; Radecki, J.; Radecka, H. Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β Peptide. Sensors 2011, 11, 4030-4042.

AMA Style

Kraziński BE, Radecki J, Radecka H. Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β Peptide. Sensors. 2011; 11(4):4030-4042.

Chicago/Turabian Style

Kraziński, Bartłomiej Emil; Radecki, Jerzy; Radecka, Hanna. 2011. "Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β Peptide." Sensors 11, no. 4: 4030-4042.

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