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Sensors 2011, 11(2), 2001-2012; doi:10.3390/s110202001

Fabrication of a Microbial Biosensor Based on QD-MWNT Supports by a One-Step Radiation Reaction and Detection of Phenolic Compounds in Red Wines

Department of Chemistry, Hannam University, Daejeon 305-811, Korea
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Author to whom correspondence should be addressed.
Received: 8 December 2010 / Revised: 4 January 2011 / Accepted: 6 February 2011 / Published: 8 February 2011
(This article belongs to the Section Biosensors)
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Abstract

An Acaligense sp.-immobilized biosensor was fabricated based on QD-MWNT composites as an electron transfer mediator and a microbe immobilization support by a one-step radiation reaction and used for sensing phenolic compounds in commercial red wines. First, a quantum dot-modified multi-wall carbon nanotube (QD-MWNT) composite was prepared in the presence of MWNT by a one-step radiation reaction in an aqueous solution at room temperature. The successful preparation of the QD-MWNT composite was confirmed by XPS, TEM, and elemental analysis. Second, the microbial biosensor was fabricated by immobilization of Acaligense sp. on the surface of the composite thin film of a glassy carbon (GC) electrode, which was prepared by a hand casting method with a mixture of the previously obtained composite and Nafion solution. The sensing ranges of the microbial biosensor based on CdS-MWNT and Cu2S-MWNT supports were 0.5–5.0 mM and 0.7–10 mM for phenol in a phosphate buffer solution, respectively. Total concentration of phenolic compounds contained in commercial red wines was also determined using the prepared microbial immobilized biosensor. View Full-Text
Keywords: microbial biosensor; quantum dots; one-step radiation reaction; electron transfer supports; phenolic compounds; red wines microbial biosensor; quantum dots; one-step radiation reaction; electron transfer supports; phenolic compounds; red wines
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MDPI and ACS Style

Kim, S.-K.; Kwen, H.-D.; Choi, S.-H. Fabrication of a Microbial Biosensor Based on QD-MWNT Supports by a One-Step Radiation Reaction and Detection of Phenolic Compounds in Red Wines. Sensors 2011, 11, 2001-2012.

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