Next Article in Journal
On the Biomimetic Design of Agile-Robot Legs
Previous Article in Journal
Modeling and Analysis of an Energy-Efficient Mobility Management Scheme in IP-Based Wireless Networks
Article Menu

Export Article

Open AccessArticle
Sensors 2011, 11(12), 11295-11304; doi:10.3390/s111211295

Protein Binding Detection Using On-Chip Silicon Gratings

Department of Electrical and Computer Engineering, the University of Western Ontario, London, ON N6A 5B9, Canada
Author to whom correspondence should be addressed.
Received: 18 October 2011 / Revised: 15 November 2011 / Accepted: 18 November 2011 / Published: 28 November 2011
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1053 KB, uploaded 21 June 2014]   |  


We demonstrate a silicon gratings-based biosensor to detect functionalized protein binding on its surface. The designed silicon gratings have sensitivities up to 197 nm/RIU in detecting refractive index change and 1.61 nm per nanometer of thickness change of bio-material on the surface of silicon gratings. Functionalizing proteins on gratings surface by eliminating unspecific binding makes this device more selective and efficient. Streptavidin at a concentration of 0.016 µmol/mL was functionalized on silicon substrate and biotin of 12 µmol/mL concentration was used as a target molecule in our detection experiments. Normal transmission measurements of gratings are made in air at different stages of immobilization, bare silicon grating, after attaching streptavidin and after trapping biotin. Total shifts in resonant peak wavelength of ~15 nm in normal transmission were observed after immobilizing biotin with ~7 nm of shift in resonant peak wavelength after functionalizing streptavidin to silicon substrate.
Keywords: gratings; immobilization; silicon; biosensor; protein gratings; immobilization; silicon; biosensor; protein
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mudraboyina, A.K.; Sabarinathan, J. Protein Binding Detection Using On-Chip Silicon Gratings. Sensors 2011, 11, 11295-11304.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top