Next Article in Journal
Application of Terahertz Radiation to Soil Measurements: Initial Results
Next Article in Special Issue
Fabrication and Characterization of Electrospun Semiconductor Nanoparticle—Polyelectrolyte Ultra-Fine Fiber Composites for Sensing Applications
Previous Article in Journal
In Situ Monitoring of Temperature inside Lithium-Ion Batteries by Flexible Micro Temperature Sensors
Previous Article in Special Issue
Biosensing with Quantum Dots: A Microfluidic Approach
Sensors 2011, 11(10), 9951-9972; doi:10.3390/s111009951
Article

Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications

1
,
1,*  and 2
1 Department of Metallurgical and Materials Engineering, School of Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte/MG, 31.270-901, Brazil 2 Department of Physics, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte/MG, 31.270-901, Brazil
* Author to whom correspondence should be addressed.
Received: 22 September 2011 / Revised: 11 October 2011 / Accepted: 17 October 2011 / Published: 21 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [1139 KB, uploaded 21 June 2014]   |  

Abstract

In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.
Keywords: hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Mansur, A.; Mansur, H.; González, J. Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications. Sensors 2011, 11, 9951-9972.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert