Next Article in Journal
Application of Terahertz Radiation to Soil Measurements: Initial Results
Next Article in Special Issue
Fabrication and Characterization of Electrospun Semiconductor Nanoparticle—Polyelectrolyte Ultra-Fine Fiber Composites for Sensing Applications
Previous Article in Journal
In Situ Monitoring of Temperature inside Lithium-Ion Batteries by Flexible Micro Temperature Sensors
Previous Article in Special Issue
Biosensing with Quantum Dots: A Microfluidic Approach
Sensors 2011, 11(10), 9951-9972; doi:10.3390/s111009951

Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications

1,*  and 2
Received: 22 September 2011 / Revised: 11 October 2011 / Accepted: 17 October 2011 / Published: 21 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [1139 KB, uploaded 21 June 2014]   |   Browse Figures


In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.
Keywords: hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Mansur, A.; Mansur, H.; González, J. Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications. Sensors 2011, 11, 9951-9972.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert