Sensors 2011, 11(10), 9951-9972; doi:10.3390/s111009951
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications
1
Department of Metallurgical and Materials Engineering, School of Engineering, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte/MG, 31.270-901, Brazil
2
Department of Physics, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte/MG, 31.270-901, Brazil
*
Author to whom correspondence should be addressed.
Received: 22 September 2011 / Revised: 11 October 2011 / Accepted: 17 October 2011 / Published: 21 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
Abstract
In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.Keywords:
hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor
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MDPI and ACS Style
Mansur, A.; Mansur, H.; González, J. Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications. Sensors 2011, 11, 9951-9972.