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Biosensing with Quantum Dots: A Microfluidic Approach
Sensors 2011, 11(10), 9951-9972; doi:10.3390/s111009951
Article

Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications

1
, 1,*  and 2
Received: 22 September 2011; in revised form: 11 October 2011 / Accepted: 17 October 2011 / Published: 21 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
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Abstract: In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.
Keywords: hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor hybrids; quantum dots; functionalized polymer; enzyme sensor; colloids; nanossensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mansur, A.; Mansur, H.; González, J. Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications. Sensors 2011, 11, 9951-9972.

AMA Style

Mansur A, Mansur H, González J. Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications. Sensors. 2011; 11(10):9951-9972.

Chicago/Turabian Style

Mansur, Alexandra; Mansur, Herman; González, Juan. 2011. "Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications." Sensors 11, no. 10: 9951-9972.


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