Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2011, 11(10), 9732-9763; doi:10.3390/s111009732
Review

Biosensing with Quantum Dots: A Microfluidic Approach

,
,
,
 and *
Received: 1 September 2011; in revised form: 4 October 2011 / Accepted: 17 October 2011 / Published: 18 October 2011
(This article belongs to the Special Issue Sensing with Quantum Dots)
View Full-Text   |   Download PDF [12900 KB, uploaded 21 June 2014]   |   Browse Figures
Abstract: Semiconductor quantum dots (QDs) have served as the basis for signal development in a variety of biosensing technologies and in applications using bioprobes. The use of QDs as physical platforms to develop biosensors and bioprobes has attracted considerable interest. This is largely due to the unique optical properties of QDs that make them excellent choices as donors in fluorescence resonance energy transfer (FRET) and well suited for optical multiplexing. The large majority of QD-based bioprobe and biosensing technologies that have been described operate in bulk solution environments, where selective binding events at the surface of QDs are often associated with relatively long periods to reach a steady-state signal. An alternative approach to the design of biosensor architectures may be provided by a microfluidic system (MFS). A MFS is able to integrate chemical and biological processes into a single platform and allows for manipulation of flow conditions to achieve, by sample transport and mixing, reaction rates that are not entirely diffusion controlled. Integrating assays in a MFS provides numerous additional advantages, which include the use of very small amounts of reagents and samples, possible sample processing before detection, ultra-high sensitivity, high throughput, short analysis time, and in situ monitoring. Herein, a comprehensive review is provided that addresses the key concepts and applications of QD-based microfluidic biosensors with an added emphasis on how this combination of technologies provides for innovations in bioassay designs. Examples from the literature are used to highlight the many advantages of biosensing in a MFS and illustrate the versatility that such a platform offers in the design strategy.
Keywords: biosensor; quantum dots; microfluidics; fluorescence resonance energy transfer; immobilization; nucleic acids; multiplexing; diagnostics; biomarkers biosensor; quantum dots; microfluidics; fluorescence resonance energy transfer; immobilization; nucleic acids; multiplexing; diagnostics; biomarkers
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Vannoy, C.H.; Tavares, A.J.; Noor, M.O.; Uddayasankar, U.; Krull, U.J. Biosensing with Quantum Dots: A Microfluidic Approach. Sensors 2011, 11, 9732-9763.

AMA Style

Vannoy CH, Tavares AJ, Noor MO, Uddayasankar U, Krull UJ. Biosensing with Quantum Dots: A Microfluidic Approach. Sensors. 2011; 11(10):9732-9763.

Chicago/Turabian Style

Vannoy, Charles H.; Tavares, Anthony J.; Noor, M. Omair; Uddayasankar, Uvaraj; Krull, Ulrich J. 2011. "Biosensing with Quantum Dots: A Microfluidic Approach." Sensors 11, no. 10: 9732-9763.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert