Detection of Single Molecules Illuminated by a Light-Emitting Diode
AbstractOptical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this paper we demonstrate optical detection of single molecules on a wide-field microscope with an illumination based on a commercially available, green light-emitting diode. The results are directly compared with laser illumination in the same experimental configuration. The setup and the limiting factors, such as light transfer to the sample, spectral filtering and the resulting signal-to-noise ratio are discussed. A theoretical and an experimental approach to estimate these parameters are presented. The results can be adapted to other single emitter and illumination schemes.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Gerhardt, I.; Mai, L.; Lamas-Linares, A.; Kurtsiefer, C. Detection of Single Molecules Illuminated by a Light-Emitting Diode. Sensors 2011, 11, 905-916.
Gerhardt I, Mai L, Lamas-Linares A, Kurtsiefer C. Detection of Single Molecules Illuminated by a Light-Emitting Diode. Sensors. 2011; 11(1):905-916.Chicago/Turabian Style
Gerhardt, Ilja; Mai, Lijian; Lamas-Linares, Antìa; Kurtsiefer, Christian. 2011. "Detection of Single Molecules Illuminated by a Light-Emitting Diode." Sensors 11, no. 1: 905-916.