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Sensors 2011, 11(1), 905-916; doi:10.3390/s110100905
Article

Detection of Single Molecules Illuminated by a Light-Emitting Diode

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Received: 8 December 2010; in revised form: 11 January 2011 / Accepted: 12 January 2011 / Published: 14 January 2011
(This article belongs to the Special Issue 10 Years Sensors - A Decade of Publishing)
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Abstract: Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this paper we demonstrate optical detection of single molecules on a wide-field microscope with an illumination based on a commercially available, green light-emitting diode. The results are directly compared with laser illumination in the same experimental configuration. The setup and the limiting factors, such as light transfer to the sample, spectral filtering and the resulting signal-to-noise ratio are discussed. A theoretical and an experimental approach to estimate these parameters are presented. The results can be adapted to other single emitter and illumination schemes.
Keywords: single molecules; fluorescence microscopy; light-emitting diode; LED; signal to noise ratio; single photon detection single molecules; fluorescence microscopy; light-emitting diode; LED; signal to noise ratio; single photon detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gerhardt, I.; Mai, L.; Lamas-Linares, A.; Kurtsiefer, C. Detection of Single Molecules Illuminated by a Light-Emitting Diode. Sensors 2011, 11, 905-916.

AMA Style

Gerhardt I, Mai L, Lamas-Linares A, Kurtsiefer C. Detection of Single Molecules Illuminated by a Light-Emitting Diode. Sensors. 2011; 11(1):905-916.

Chicago/Turabian Style

Gerhardt, Ilja; Mai, Lijian; Lamas-Linares, Antìa; Kurtsiefer, Christian. 2011. "Detection of Single Molecules Illuminated by a Light-Emitting Diode." Sensors 11, no. 1: 905-916.


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