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Sensors 2011, 11(1), 180-206; doi:10.3390/s110100180
Review

In Vivo Bioluminescent Imaging (BLI): Noninvasive Visualization and Interrogation of Biological Processes in Living Animals

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*
The Center for Environmental Biotechnology, 676 Dabney Hall, The University of Tennessee, Knoxville, TN 37996, USA
* Author to whom correspondence should be addressed.
Received: 14 November 2010 / Revised: 6 December 2010 / Accepted: 23 December 2010 / Published: 28 December 2010
(This article belongs to the Special Issue Sensors in Biomechanics and Biomedicine)
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Abstract

In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.
Keywords: bacterial luciferase; bioluminescent imaging; BLI; firefly luciferase; Lux; Luc bacterial luciferase; bioluminescent imaging; BLI; firefly luciferase; Lux; Luc
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Close, D.M.; Xu, T.; Sayler, G.S.; Ripp, S. In Vivo Bioluminescent Imaging (BLI): Noninvasive Visualization and Interrogation of Biological Processes in Living Animals. Sensors 2011, 11, 180-206.

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