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Sensors 2010, 10(6), 5859-5871; doi:10.3390/s100605859

Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element

Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Department of Laboratory Medicine, the General Navy Hospital, Beijing 100037, China
Author to whom correspondence should be addressed.
Received: 30 March 2010 / Revised: 20 April 2010 / Accepted: 15 May 2010 / Published: 9 June 2010
(This article belongs to the Section Biosensors)
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The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10–240 μg/L) compared to that of the aptamer (2.5–200 μg/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.
Keywords: quartz crystal microbalance; biosensor; aptamer; antibody; IgE quartz crystal microbalance; biosensor; aptamer; antibody; IgE

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yao, C.; Zhu, T.; Qi, Y.; Zhao, Y.; Xia, H.; Fu, W. Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element. Sensors 2010, 10, 5859-5871.

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