Next Article in Journal
An RFID-Based Intelligent Vehicle Speed Controller Using Active Traffic Signals
Previous Article in Journal
Microfabricated Thin Film Impedance Sensor & AC Impedance Measurements
Sensors 2010, 10(6), 5859-5871; doi:10.3390/s100605859
Article

Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element

1
, 2
, 3, 1
, 1
 and 1,*
Received: 30 March 2010; in revised form: 20 April 2010 / Accepted: 15 May 2010 / Published: 9 June 2010
(This article belongs to the Section Biosensors)
Download PDF [295 KB, updated 21 June 2014; original version uploaded 21 June 2014]
Abstract: The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10–240 μg/L) compared to that of the aptamer (2.5–200 μg/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.
Keywords: quartz crystal microbalance; biosensor; aptamer; antibody; IgE quartz crystal microbalance; biosensor; aptamer; antibody; IgE
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Yao, C.; Zhu, T.; Qi, Y.; Zhao, Y.; Xia, H.; Fu, W. Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element. Sensors 2010, 10, 5859-5871.

AMA Style

Yao C, Zhu T, Qi Y, Zhao Y, Xia H, Fu W. Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element. Sensors. 2010; 10(6):5859-5871.

Chicago/Turabian Style

Yao, Chunyan; Zhu, Tangyou; Qi, Yongzhi; Zhao, Yuhui; Xia, Han; Fu, Weiling. 2010. "Development of a Quartz Crystal Microbalance Biosensor with Aptamers as Bio-recognition Element." Sensors 10, no. 6: 5859-5871.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert