Sensors 2010, 10(6), 5798-5808; https://doi.org/10.3390/s100605798
Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator
1
Department of Biological Engineering, 240D Bond Life Sciences Center, 1201 East Rollins Street, University of Missouri, Columbia, MO 65211, USA
2
Department of Biomedical Engineering, 2158 Lurie Biomedical Engineering Building, 1101 Beal Ave., University of Michigan, Ann Arbor, MI 48109, USA
*
Author to whom correspondence should be addressed.
Received: 3 April 2010 / Revised: 16 May 2010 / Accepted: 1 June 2010 / Published: 8 June 2010
(This article belongs to the Section Biosensors)
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Abstract
We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression. View Full-TextKeywords:
HIV; CD4; CD8; ring resonator; optical biosensor; label free
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Gohring, J.T.; Fan, X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors 2010, 10, 5798-5808.