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Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator
Department of Biological Engineering, 240D Bond Life Sciences Center, 1201 East Rollins Street, University of Missouri, Columbia, MO 65211, USA
Department of Biomedical Engineering, 2158 Lurie Biomedical Engineering Building, 1101 Beal Ave., University of Michigan, Ann Arbor, MI 48109, USA
* Author to whom correspondence should be addressed.
Received: 3 April 2010; in revised form: 16 May 2010 / Accepted: 1 June 2010 / Published: 8 June 2010
Abstract: We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
Keywords: HIV; CD4; CD8; ring resonator; optical biosensor; label free
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Cite This Article
MDPI and ACS Style
Gohring, J.T.; Fan, X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors 2010, 10, 5798-5808.
Gohring JT, Fan X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors. 2010; 10(6):5798-5808.
Gohring, John T.; Fan, Xudong. 2010. "Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator." Sensors 10, no. 6: 5798-5808.