Next Article in Journal
Power Consumption Analysis of Operating Systems for Wireless Sensor Networks
Previous Article in Journal
Sensor Systems for Prognostics and Health Management
Sensors 2010, 10(6), 5798-5808; doi:10.3390/s100605798

Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator

1 Department of Biological Engineering, 240D Bond Life Sciences Center, 1201 East Rollins Street, University of Missouri, Columbia, MO 65211, USA 2 Department of Biomedical Engineering, 2158 Lurie Biomedical Engineering Building, 1101 Beal Ave., University of Michigan, Ann Arbor, MI 48109, USA
* Author to whom correspondence should be addressed.
Received: 3 April 2010 / Revised: 16 May 2010 / Accepted: 1 June 2010 / Published: 8 June 2010
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [252 KB, uploaded 21 June 2014]   |   Browse Figures


We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
Keywords: HIV; CD4; CD8; ring resonator; optical biosensor; label free HIV; CD4; CD8; ring resonator; optical biosensor; label free
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Gohring, J.T.; Fan, X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors 2010, 10, 5798-5808.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert