Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator
AbstractWe have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
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Gohring, J.T.; Fan, X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors 2010, 10, 5798-5808.
Gohring JT, Fan X. Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator. Sensors. 2010; 10(6):5798-5808.Chicago/Turabian Style
Gohring, John T.; Fan, Xudong. 2010. "Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator." Sensors 10, no. 6: 5798-5808.