Sensors 2010, 10(5), 4739-4755; doi:10.3390/s100504739
Article

Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins

1,* email, 1email, 2email and 3email
Received: 5 March 2010; in revised form: 22 April 2010 / Accepted: 27 April 2010 / Published: 10 May 2010
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in the UK)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The fabrication of novel uranyl (UO22+) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO22+ ions and showed minor interference from Ni2+, Cs+, Cd2+ and Co2+. Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO22+ binding, showing that both moieties are involved in the recognition to UO22+.
Keywords: S-layer; surface layer; protein biosensor; uranium; uranyl; metal ion; sequestering; impedance spectroscopy
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MDPI and ACS Style

Conroy, D.J.; Millner, P.A.; Stewart, D.I.; Pollmann, K. Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins. Sensors 2010, 10, 4739-4755.

AMA Style

Conroy DJ, Millner PA, Stewart DI, Pollmann K. Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins. Sensors. 2010; 10(5):4739-4755.

Chicago/Turabian Style

Conroy, David J.R.; Millner, Paul A.; Stewart, Douglas I.; Pollmann, Katrin. 2010. "Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins." Sensors 10, no. 5: 4739-4755.

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