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Sensors 2010, 10(5), 4739-4755; doi:10.3390/s100504739

Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins

1
Biosensors and Biocatalysis Group, Institute of Membranes and Systems Biology, University of Leeds, Leeds, LS2 9JT, UK
2
School of Civil Engineering, University of Leeds, Leeds LS2 9JT, UK
3
Institute of Radiochemistry, Forschungszentrum Dresden, Rossendorf, Germany
*
Author to whom correspondence should be addressed.
Received: 5 March 2010 / Revised: 22 April 2010 / Accepted: 27 April 2010 / Published: 10 May 2010
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in the UK)

Abstract

The fabrication of novel uranyl (UO22+) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO22+ ions and showed minor interference from Ni2+, Cs+, Cd2+ and Co2+. Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO22+ binding, showing that both moieties are involved in the recognition to UO22+.
Keywords: S-layer; surface layer; protein biosensor; uranium; uranyl; metal ion; sequestering; impedance spectroscopy S-layer; surface layer; protein biosensor; uranium; uranyl; metal ion; sequestering; impedance spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Conroy, D.J.; Millner, P.A.; Stewart, D.I.; Pollmann, K. Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins. Sensors 2010, 10, 4739-4755.

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