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Design Strategies for Aptamer-Based Biosensors
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
Suzhou Institute of Biomedical Engineering Technology, Chinese Academy of Science, Suzhou 215163, China
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
School of Biotechnology and the Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 11 February 2010; in revised form: 1 April 2010 / Accepted: 4 April 2010 / Published: 4 May 2010
Abstract: Aptamers have been widely used as recognition elements for biosensor construction, especially in the detection of proteins or small molecule targets, and regarded as promising alternatives for antibodies in bioassay areas. In this review, we present an overview of reported design strategies for the fabrication of biosensors and classify them into four basic modes: target-induced structure switching mode, sandwich or sandwich-like mode, target-induced dissociation/displacement mode and competitive replacement mode. In view of the unprecedented advantages brought about by aptamers and smart design strategies, aptamer-based biosensors are expected to be one of the most promising devices in bioassay related applications.
Keywords: aptamer; biosensor; design strategy
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MDPI and ACS Style
Han, K.; Liang, Z.; Zhou, N. Design Strategies for Aptamer-Based Biosensors. Sensors 2010, 10, 4541-4557.
Han K, Liang Z, Zhou N. Design Strategies for Aptamer-Based Biosensors. Sensors. 2010; 10(5):4541-4557.
Han, Kun; Liang, Zhiqiang; Zhou, Nandi. 2010. "Design Strategies for Aptamer-Based Biosensors." Sensors 10, no. 5: 4541-4557.