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Diversity 2016, 8(1), 1; doi:10.3390/d8010001

Can Theory Improve the Scope of Quantitative Metazoan Metabarcoding?

1
Department of Genetics and Odum School of Ecology, University of Georgia, Athens, GA 30602, USA
2
Odum School of Ecology, University of Georgia, Athens, GA 30602, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Wink
Received: 2 November 2015 / Revised: 21 December 2015 / Accepted: 22 December 2015 / Published: 29 December 2015
(This article belongs to the Special Issue DNA Barcoding and Biodiversity Research)
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Abstract

Using high-throughput sequencing approaches to quantify biodiversity has a number of hurdles, in particular that the number of reads for a given taxon may not be proportional to the number of individuals of that taxon in a sample. Here, we consider whether summary statistics generated in the course of population genetic analyses (such as estimates of haplotype diversity and mutation rate) may be useful in reverse inference of the number of individuals input to an assay. Although our results show that these statistics—combined with the observed number of segregating sites and number of haplotypes in the assay—may be informative, there remain significant concerns about the ability to “metabarcode” a sample and infer relative species abundance. View Full-Text
Keywords: biodiversity; species diversity; population genetics; summary statistics; coalescent theory; metabarcoding biodiversity; species diversity; population genetics; summary statistics; coalescent theory; metabarcoding
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wares, J.P.; Pappalardo, P. Can Theory Improve the Scope of Quantitative Metazoan Metabarcoding? Diversity 2016, 8, 1.

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