Diversity 2013, 5(3), 581-612; doi:10.3390/d5030581

Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology

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Received: 6 May 2013; in revised form: 21 June 2013 / Accepted: 25 June 2013 / Published: 2 August 2013
(This article belongs to the Special Issue Microbial Ecology and Diversity)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Soil microbial communities play an important role in plant health and soil quality. Researchers have developed a wide range of methods for studying the structure, diversity, and activity of microbes to better understand soil biology and plant-microbe interactions. Functional microbiological analyses of the rhizosphere have given new insights into the role of microbial communities in plant nutrition and plant protection against diseases. In this review, we present the most commonly used traditional as well as new culture-independent molecular methods to assess the diversity and function of soil microbial communities. Furthermore, we discuss advantages and disadvantages of these techniques and provide a perspective on emerging technologies for soil microbial community profiling.
Keywords: DNA microarrays; fatty acid analysis; molecular fingerprinting methods; next generation sequencing; plant-microbe interactions; rhizosphere biology; soil quality; soil microbial communities
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MDPI and ACS Style

Rincon-Florez, V.A.; Carvalhais, L.C.; Schenk, P.M. Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology. Diversity 2013, 5, 581-612.

AMA Style

Rincon-Florez VA, Carvalhais LC, Schenk PM. Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology. Diversity. 2013; 5(3):581-612.

Chicago/Turabian Style

Rincon-Florez, Vivian A.; Carvalhais, Lilia C.; Schenk, Peer M. 2013. "Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology." Diversity 5, no. 3: 581-612.

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