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Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology
Plant-Microbe Interactions Group, School of Agriculture and Food Sciences, the University of Queensland, Brisbane, QLD 4072, Australia
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Received: 6 May 2013; in revised form: 21 June 2013 / Accepted: 25 June 2013 / Published: 2 August 2013
Abstract: Soil microbial communities play an important role in plant health and soil quality. Researchers have developed a wide range of methods for studying the structure, diversity, and activity of microbes to better understand soil biology and plant-microbe interactions. Functional microbiological analyses of the rhizosphere have given new insights into the role of microbial communities in plant nutrition and plant protection against diseases. In this review, we present the most commonly used traditional as well as new culture-independent molecular methods to assess the diversity and function of soil microbial communities. Furthermore, we discuss advantages and disadvantages of these techniques and provide a perspective on emerging technologies for soil microbial community profiling.
Keywords: DNA microarrays; fatty acid analysis; molecular fingerprinting methods; next generation sequencing; plant-microbe interactions; rhizosphere biology; soil quality; soil microbial communities
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Rincon-Florez, V.A.; Carvalhais, L.C.; Schenk, P.M. Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology. Diversity 2013, 5, 581-612.
Rincon-Florez VA, Carvalhais LC, Schenk PM. Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology. Diversity. 2013; 5(3):581-612.
Rincon-Florez, Vivian A.; Carvalhais, Lilia C.; Schenk, Peer M. 2013. "Culture-Independent Molecular Tools for Soil and Rhizosphere Microbiology." Diversity 5, no. 3: 581-612.