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Int. J. Mol. Sci. 2008, 9(9), 1676-1684; doi:10.3390/ijms9091676

Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]

Department of Biochemistry, State University of Maringá. PR, Brasil
Author to whom correspondence should be addressed.
Received: 27 June 2008 / Revised: 8 August 2008 / Accepted: 29 August 2008 / Published: 2 September 2008
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The efficacy of different concentrations of aqueous neem leaf extract (3.12 to 50 mg/mL) on growth and citrinin production in three isolates of Penicillium citrinum was investigated under laboratory conditions. Mycotoxin production by the isolates was suppressed, depending on the concentration of the plant extract added to culture media at the time of spore inoculation. Citrinin production in fungal mycelia grown for 21 days in culture media containing 3.12 mg/mL of the aqueous extract of neem leaf was inhibited by approximately 80% in three isolates of P. citrinum. High-performance liquid chromatography was performed to confirm the spectrophotometric results. Vegetative growth was assessed, but neem extract failed to inhibit it. Neem leaf extract showed inhibition of toxin production without retardation in fungal mycelia growth.
Keywords: Penicillium citrinum; citrinin inhibition; Azadirachta indica; neem Penicillium citrinum; citrinin inhibition; Azadirachta indica; neem
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mossini, S.A.G.; Kemmelmeier, C. Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]. Int. J. Mol. Sci. 2008, 9, 1676-1684.

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