Next Article in Journal
Integration of Motor Proteins – Towards an ATP Fueled Soft Actuator
Next Article in Special Issue
Are the Genes nadA and norB Involved in Formation of Aflatoxin G1?
Previous Article in Journal
Pyrolysis of Softwood Carbohydrates in a Fluidized Bed Reactor
Previous Article in Special Issue
The Use of Isolated Human Lymphocytes in Mycotoxin Cytotoxicity Testing
Int. J. Mol. Sci. 2008, 9(9), 1676-1684; doi:10.3390/ijms9091676

Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]

 and *
Department of Biochemistry, State University of Maringá. PR, Brasil
* Author to whom correspondence should be addressed.
Received: 27 June 2008 / Revised: 8 August 2008 / Accepted: 29 August 2008 / Published: 2 September 2008
View Full-Text   |   Download PDF [627 KB, uploaded 19 June 2014]   |   Browse Figures


The efficacy of different concentrations of aqueous neem leaf extract (3.12 to 50 mg/mL) on growth and citrinin production in three isolates of Penicillium citrinum was investigated under laboratory conditions. Mycotoxin production by the isolates was suppressed, depending on the concentration of the plant extract added to culture media at the time of spore inoculation. Citrinin production in fungal mycelia grown for 21 days in culture media containing 3.12 mg/mL of the aqueous extract of neem leaf was inhibited by approximately 80% in three isolates of P. citrinum. High-performance liquid chromatography was performed to confirm the spectrophotometric results. Vegetative growth was assessed, but neem extract failed to inhibit it. Neem leaf extract showed inhibition of toxin production without retardation in fungal mycelia growth.
Keywords: Penicillium citrinum; citrinin inhibition; Azadirachta indica; neem Penicillium citrinum; citrinin inhibition; Azadirachta indica; neem
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Mossini, S.A.G.; Kemmelmeier, C. Inhibition of Citrinin Production in Penicillium citrinum Cultures by Neem [Azadirachta indica A. Juss (Meliaceae)]. Int. J. Mol. Sci. 2008, 9, 1676-1684.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert