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Int. J. Mol. Sci. 2008, 9(6), 943-950; doi:10.3390/ijms9060943

Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1)

1
College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Place, Saskatoon, Saskatchewan, S7N 5C9, Canada
2
Department of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada
*
Author to whom correspondence should be addressed.
Received: 2 April 2008 / Revised: 28 May 2008 / Accepted: 2 June 2008 / Published: 2 June 2008
(This article belongs to the Special Issue Protein Crystallography)
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Abstract

Through alternative promoter usage, human retinoblastoma protein-interacting zinc finger gene RIZ encodes two different protein products, RIZ1 and RIZ2, which have been identified to be a tumor suppressor and a proto-oncoprotein, respectively. Structurally, the two protein products share the same amino acid sequences except that RIZ2 lacks an N-terminal PR domain with methyltransferase activity. Previous studies have shown that over-expression of RIZ2 is usually associated with depressed RIZ1 expression in different human cancers. It is generally believed that RIZ1 and RIZ2 regulate normal cell division and function using a “Yin-Yang” fashion and the PR domain is responsible for the tumor suppressing activity of RIZ1. In order to better understand the biological functions of the PR domain by determining its three-dimensional crystal structure, we expressed, purified and crystallized a construct of the PR domain (amino acid residues 13-190) in this study. The maximum size of the needle-shaped crystals was approximately 0.20 x 0.01 x 0.01 mm.
Keywords: tumor suppressor; Yin-Yang regulation; zinc finger; affinity chromatography; protein crystallization tumor suppressor; Yin-Yang regulation; zinc finger; affinity chromatography; protein crystallization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sun, W.; Geyer, C.R.; Yang, J. Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1). Int. J. Mol. Sci. 2008, 9, 943-950.

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