Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2008, 9(6), 943-950; doi:10.3390/ijms9060943
Article

Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1)

1
, 2
 and 1,*
Received: 2 April 2008; in revised form: 28 May 2008 / Accepted: 2 June 2008 / Published: 2 June 2008
(This article belongs to the Special Issue Protein Crystallography)
Download PDF [367 KB, uploaded 19 June 2014]
Abstract: Through alternative promoter usage, human retinoblastoma protein-interacting zinc finger gene RIZ encodes two different protein products, RIZ1 and RIZ2, which have been identified to be a tumor suppressor and a proto-oncoprotein, respectively. Structurally, the two protein products share the same amino acid sequences except that RIZ2 lacks an N-terminal PR domain with methyltransferase activity. Previous studies have shown that over-expression of RIZ2 is usually associated with depressed RIZ1 expression in different human cancers. It is generally believed that RIZ1 and RIZ2 regulate normal cell division and function using a “Yin-Yang” fashion and the PR domain is responsible for the tumor suppressing activity of RIZ1. In order to better understand the biological functions of the PR domain by determining its three-dimensional crystal structure, we expressed, purified and crystallized a construct of the PR domain (amino acid residues 13-190) in this study. The maximum size of the needle-shaped crystals was approximately 0.20 x 0.01 x 0.01 mm.
Keywords: tumor suppressor; Yin-Yang regulation; zinc finger; affinity chromatography; protein crystallization tumor suppressor; Yin-Yang regulation; zinc finger; affinity chromatography; protein crystallization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sun, W.; Geyer, C.R.; Yang, J. Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1). Int. J. Mol. Sci. 2008, 9, 943-950.

AMA Style

Sun W, Geyer CR, Yang J. Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1). International Journal of Molecular Sciences. 2008; 9(6):943-950.

Chicago/Turabian Style

Sun, Wanpeng; Geyer, C. R.; Yang, Jian. 2008. "Cloning, Expression, Purification and Crystallization of the PR Domain of Human Retinoblastoma Protein-Binding Zinc Finger Protein 1 (RIZ1)." Int. J. Mol. Sci. 9, no. 6: 943-950.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert