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Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
Laboratório de Biologia Estrutural e Cristalografia, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970, Campinas-SP, Brazil
Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP, Brazil
Laboratório de Química de Macromoléculas, UNESP/CLP, São Vicente-SP, Brazil
* Author to whom correspondence should be addressed.
Received: 4 February 2008; in revised form: 6 March 2008 / Accepted: 22 March 2008 / Published: 8 May 2008
Abstract: BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 °A resolution and 1.9 °A resolution for crystals belonging to the space group P212121 (a = 48:4 A° , b = 65:3 A° , c = 84:3 A° ) and space group P3121 (a = b = 55:7 A° , c = 127:9 A° ), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII.
Keywords: Lys49-PLA2; Bothrops jararacussu; platelet aggregation; phospholipase crystallographic analysis
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Dos Santos, M.L.; Fagundes, F.H.R.; Teixeira, B.R.F.; Toyama, M.H.; Aparicio, R. Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu. Int. J. Mol. Sci. 2008, 9, 736-750.
Dos Santos ML, Fagundes FHR, Teixeira BRF, Toyama MH, Aparicio R. Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu. International Journal of Molecular Sciences. 2008; 9(5):736-750.
Dos Santos, Marcelo L.; Fagundes, Fábio H.R.; Teixeira, Bruno R.F.; Toyama, Marcos H.; Aparicio, Ricardo. 2008. "Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu." Int. J. Mol. Sci. 9, no. 5: 736-750.