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Int. J. Mol. Sci. 2008, 9(4), 606-614; doi:10.3390/ijms9040606
Article

Quantification of Phenolic Constituents and Antioxidant Activity of Pterodon emarginatus Vogel Seeds

1
,
2
 and
1,*
1 Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brasil 2 Departamento Farmacêutico, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brasil
* Author to whom correspondence should be addressed.
Received: 16 October 2007 / Revised: 18 April 2008 / Accepted: 18 April 2008 / Published: 18 April 2008
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Abstract

In the present study the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents and the antioxidant activity of Pterodon emarginatus seeds were investigated in several samples prepared with different extraction procedures: essential oil (EO) using a Clevenger-type apparatus; hexanic (HF), ethyl acetate (EAF), buthanolic (BF) and methanolic (MF) fractions using Soxhlet extraction, and extracts (1 g/extract) obtained from different methods: reflux 80°C/30 min, ultrasound/30 min, static maceration/48 h and heating plate 100°C/45 min. These extracts were prepared using water or ethanol/water at 30:70 v/v, 50:50 v/v or 70:30 v/v. Antioxidant activity [2,2-diphenyl-2- picrylhydrazyl hydrate (DPPH)] was tested only in the fractions obtained from Soxhlet extraction. The extract obtained from reflux using ethanol/water (70:30, v/v) showed the highest phenolic constituents level. The EAF, BF and MF showed DPPH scavenging activities with IC50=163.22, 18.89 and 10.15 μg/ml, respectively.
Keywords: Pterodon emarginatus; Leguminosae; phenolic constituents; DPPH. Pterodon emarginatus; Leguminosae; phenolic constituents; DPPH.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Dutra, R.C.; Leite, M.N.; Barbosa, N.R. Quantification of Phenolic Constituents and Antioxidant Activity of Pterodon emarginatus Vogel Seeds. Int. J. Mol. Sci. 2008, 9, 606-614.

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