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Int. J. Mol. Sci. 2008, 9(3), 299-315; doi:10.3390/ijms9030299

RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras

2, 1
Received: 13 December 2007 / Revised: 29 February 2008 / Accepted: 29 February 2008 / Published: 12 March 2008
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
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The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3’-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum.
Keywords: modified siRNA; RNA interference; RNA-PNA chimeras; Peptide Nucleic Acids; RNA. modified siRNA; RNA interference; RNA-PNA chimeras; Peptide Nucleic Acids; RNA.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Potenza, N.; Moggio, L.; Milano, G.; Salvatore, V.; Di Blasio, B.; Russo, A.; Messere, A. RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras. Int. J. Mol. Sci. 2008, 9, 299-315.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert