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RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras
Department of Life Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy
Department of Environmental Sciences, Second University of Naples, Via Vivaldi 43, I-81100 Caserta, Italy
* Author to whom correspondence should be addressed.
Received: 13 December 2007; in revised form: 29 February 2008 / Accepted: 29 February 2008 / Published: 12 March 2008
Abstract: The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3’-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum.
Keywords: modified siRNA; RNA interference; RNA-PNA chimeras; Peptide Nucleic Acids; RNA.
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Potenza, N.; Moggio, L.; Milano, G.; Salvatore, V.; Di Blasio, B.; Russo, A.; Messere, A. RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras. Int. J. Mol. Sci. 2008, 9, 299-315.
Potenza N, Moggio L, Milano G, Salvatore V, Di Blasio B, Russo A, Messere A. RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras. International Journal of Molecular Sciences. 2008; 9(3):299-315.
Potenza, Nicoletta; Moggio, Loredana; Milano, Giovanna; Salvatore, Vincenzo; Di Blasio, Benedetto; Russo, Aniello; Messere, Anna. 2008. "RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras." Int. J. Mol. Sci. 9, no. 3: 299-315.