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Int. J. Mol. Sci. 2008, 9(3), 247-257; doi:10.3390/ijms9030247

Expression, Characterization and Synergistic Interactions of Myxobacter Sp. AL-1 Cel9 and Cel48 Glycosyl Hydrolases

1, 1, 1, 1, 2 and 1,*
1 Instituto de Investigación en Biología Experimental (IIBE), Facultad de Química, Universidad de Guanajuato, P.O. Box 187, Guanajuato, Gto. 36050, Mexico 2 Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Abasolo 600, Pachuca, Hgo 42000, Mexico
* Author to whom correspondence should be addressed.
Received: 2 December 2007 / Revised: 24 January 2008 / Accepted: 1 February 2008 / Published: 29 February 2008
(This article belongs to the Special Issue Biofuels R&D: Securing the Planet's Future Energy Needs)
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The soil microorganism Myxobacter Sp. AL-1 regulates in a differential manner the production of five extracellular cellulases during its life cycle. The nucleotide sequence of a cel9-cel48 cluster from the genome of this microorganism was recently obtained. Cel48 was expressed in Escherichia coli to generate a His6-Cel48 protein and the biochemical properties of the pure protein were determined. Cel48 was more efficient in degrading acid-swollen avicel (ASC) than carboxymethylcellulose (CMC). On the other hand, cel9 was expressed in Bacillus subtilis from an IPTG-inducible promoter. Zymogram analysis showed that after IPTG-induction, Cel9 existed in both the cell fraction and the culture medium of B. subtilis and the secreted protein was purified to homogeneity by FPLC-ionic exchange chromatography. The exocellobiohydrolase Cel48 showed a synergism of 1.68 times with the endocellulase Cel9 during ASC degradation using an 8.1- fold excess of Cel48 over Cel9. Western blot analysis revealed that both proteins were synthesized and secreted to the culture medium of Myxobacter Sp. AL-1. These results show that the cel9-cel48 cluster encodes functional endo- and exo-acting cellulases that allows Myobacter Sp. AL-1 to hydrolyse cellulose.
Keywords: Cellulose; Cel9; Cel48 cellulases; Synergism; Myxobacter Sp. AL-1. Cellulose; Cel9; Cel48 cellulases; Synergism; Myxobacter Sp. AL-1.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Ramírez-Ramírez, N.; Romero-García, E.R.; Calderón, V.C.; Avitia, C.I.; Téllez-Valencia, A.; Pedraza-Reyes, M. Expression, Characterization and Synergistic Interactions of Myxobacter Sp. AL-1 Cel9 and Cel48 Glycosyl Hydrolases. Int. J. Mol. Sci. 2008, 9, 247-257.

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