Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody
Abstract
:1. Introduction
2. Results and Discussion
2.1 Preparation of antigen
2.2 Building of hybridoma cells
2.3 Antibody properties
2.4 Dependence on pH
2.5 Kinetics of catalytic reaction
2.6 Thermostability of the chloroperoxidase activity
3. Experimental section
3.1 Materials and Reagents
3.2 Antigen Preparation
3.3 Preparation and purification of monoclonal antibody against meso-tetra(α,α,α,α-o-phenylacetamidophenyl) porphyrin
3.4 Preparation of Fe-Porphyrin
3.5 Dependence on pH
3.6 Assay of chloroperoxidase activity
3.7 Thermostability of chloroperoxidase
Vmax (M·s−1) | Km (M) | κcat (s−1) | κcat/Km (M−1·s−1) | |
---|---|---|---|---|
Abzyme | 5.18±0.12×10−8 | 1.50±0.03×10−8 | 0.518±0.1 | 3.45±0.15×107 |
Fe porphyrin | 2.44±0.05×10−8 | 5.2±0.14×10−8 | 0.244±0.08 | 4.69±0.18×107 |
Acknowledgements
References
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Wang, F.; Huang, X.; Du, L.; Li, W.; He, H.; Qi, C. Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody. Int. J. Mol. Sci. 2007, 8, 445-454. https://doi.org/10.3390/i8050445
Wang F, Huang X, Du L, Li W, He H, Qi C. Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody. International Journal of Molecular Sciences. 2007; 8(5):445-454. https://doi.org/10.3390/i8050445
Chicago/Turabian StyleWang, Fengyang, Xueying Huang, Li Du, Weiguo Li, Hongxuan He, and Chao Qi. 2007. "Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody" International Journal of Molecular Sciences 8, no. 5: 445-454. https://doi.org/10.3390/i8050445