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Int. J. Mol. Sci. 2007, 8(11), 1165-1176; doi:10.3390/i8111165

Article

Inhibition of Acetylcholinesterase in Different Structures of the Rat Brain Following Soman Intoxication Pretreated with Huperzine A

Jiri Bajgar ^1,* , Petr Hajek ² , Jana Karasova ¹ , Dasa Slizova ² , Otakar Krs ² , Kamil Kuca ¹ , Daniel Jun ¹ , Josef Fusek ¹  and Lukas Capek ⁴

¹ Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic ² Department of Anatomy, Medical Faculty of Charles University, Hradec Kralove, Czech Republic ³ Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic ⁴ Department of Engineering Mechanics, Faculty of Mechanical Engineering, Technical University of Liberec, Liberec, Czech Republic

* Author to whom correspondence should be addressed.

Received: 15 October 2007; in revised form: 14 November 2007 / Accepted: 15 November 2007 / Published: 26 November 2007

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

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Abstract: Acetylcholinesterase (AChE) activities in different brain parts were determinedquantitatively in rats treated with huperzine A, soman, and huperzine A followed bysoman, using histochemical and biochemical methods. Following soman intoxication (1.2x LD50, i.m.), AChE activity was decreased to 30-80% of control values depending on thebrain structure. The most sensitive area was the frontal cortex and the most relativelyresistant was ncl. ruber. Huperzine A treatment only caused a change in AChE activityvarying from 70 to 100 % of control values. In rats pretreated with huperzine A andintoxicated with soman, AChE activity was significantly higher than that observed aftersoman. In these animals, survival of rats pretreated with huperzine was observed whilethe mortality of unpretreated animals was near to 80 %. The results suggest that huperzineA is good candidate for further study for clinical use as a prophylactic drug against nerveagent poisoning.

Keywords: acetylcholinesterase; rat brain parts; soman; Huperzine A; pretreatment

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