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Int. J. Mol. Sci. 2006, 7(8), 266-273; doi:10.3390/i7080266

Article

Determination of Trace Level of cAMP in Locusta Migratoria Manilensis Meyen by HPLC with Fluorescence Derivation

Lujun Zhang, Huiting Zhang, Jinfang Wang and Canping Pan*

College of Sciences, China Agricultural University, Beijing 100094, China

* Author to whom correspondence should be addressed.

Received: 2 March 2006; in revised form: 30 May 2006 / Accepted: 21 July 2006 / Published: 9 August 2006

Download PDF Full-Text [80 KB, uploaded 20 June 2008 16:51 CEST]

Abstract: A sensitive and rapid method was developed for the determination of cAMP inLocusta migratoria manilensis Meyen by high-performance liquid chromatography withfluorescence detection. The cAMP was derivatized using chloroacetaldehyde and TBASbuffer/methanol was used as the mobile phase. A detection quantification of 40 fmol/mlcould be achieved when using fluorescence detection. An HPLC-MS method using DMHAas an ion-pair agent to analyze cAMP was also demonstrated. We studied the effect ofdopamine and other stimulants on cAMP levels from isolated locust central nervoussystems. The new method is well suited for the analysis of cAMP in small biologicalsamples.

Keywords: cAMP; OA; Locusta migratoria manilensis Meyen; HPLC; HPLC-MS; fluorescence derivation; DMHA; TBAS.

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