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Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads
State Key Bioelectronics Laboratory, Southeast University, Sipailou2#, Nanjing, 210096, P.R. China
Institute of Chemistry & Hydrometallurgy, Jinchuan Group Co., Ltd., Lanzhou Road, Jinchang,737100, P.R.China
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,Chinese Academy of Sciences, Beijing, 100080, P.R. China
Technical Institute of Physics and Chemistry, Chinese Academy of Science, Datun Street First 3#, Beijing, 100101, P.R. China
* Author to whom correspondence should be addressed.
Received: 26 May 2006; Accepted: 3 August 2006 / Published: 24 August 2006
Abstract: Superparamagnetic polymers (SPMP) microbead was an excellent form fornanosized magnetic particles less than 10nm to realize their potential applications in manyfields. We developed a novel modified suspension polymerization method for the productionof superparamagnetic poly (methacrylate divinylbenzene) (PMA-DVB) microbeads, withamino groups on their surface after simple modifications. We applied these SPMPmicrobeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure ofdetecting the free hCGβ in serum. It was proved to a better method compared to the ELISA,since it need half of the sample volume, simpler protocols, and the time it need wasshortened from 2 hours to 1 hour. The detection limit was 0.22 mIU•m-1, one order lowerthan the ELISA assay. Its linear range was between 0.45—185.2 mIU•m-1. And in thedetections of clinical serum samples, the related coefficient between the two methods’results was 0.955.
Keywords: superparamagnetic polymer microbeads; modified suspension polymerization; enzyme chemiluminescence immunoassay; free hCGβ; tumor marker.
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MDPI and ACS Style
Guo, X.; Guan, Y.; Yang, B.; Wang, Y.; Lan, H.; Shi, W.; Yang, Z.; Lu, Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. Int. J. Mol. Sci. 2006, 7, 274-288.
Guo X, Guan Y, Yang B, Wang Y, Lan H, Shi W, Yang Z, Lu Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. International Journal of Molecular Sciences. 2006; 7(8):274-288.
Guo, Xiaoying; Guan, Yueping; Yang, Bin; Wang, Yongning; Lan, Hualong; Shi, Wentang; Yang, Zhenghui; Lu, Zuhong. 2006. "Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads." Int. J. Mol. Sci. 7, no. 8: 274-288.