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Expression of Myosin Light Chain Kinase in Kidney of Streptozotocin-Induced Diabetic Rats
Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, Anhui, P.R.China
Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui, P.R.China
Department of Respiratory Disease, Anhui Medical University, Hefei 230032, Anhui, P.R.China
Anhui Province Key Laboratory of Genomic Research and the Key Laboratory of Gene Resource Utilization for Severe Disease (Anhui Medical University ), Ministry of Education P.R.China, Hefei 230032, Anhui, P.R.China
* Author to whom correspondence should be addressed.
Received: 2 October 2006; Accepted: 13 November 2006 / Published: 21 November 2006
Abstract: Nephropathy is one of the most common complications of diabetes mellituswhich remains incompletely understood. We reported the expression of myosin light chainkinase (MLCK) in the kidney of diabetic rats and investigated the correlation betweenMLCK and diabetic nephropathy by observing the expression of MLCK. The diabetic modelrats were induced by an intraperitoneal injection of streptozotocin (STZ) and the insulin-treated rats were subcutaneously injected with protamine zine insulin 3u/d. The kidneyswere excised and immersed in 4% polyoxymethylene after 12 weeks later. The expression ofMLCK was analyzed by immunohistochemical staining and Western blot.Immunohistochemical analysis and Western blot assay indicated that the MLCK expressionwas higher in kidney of diabetic rats than that in control and it was decreased in kidney ofinsulin-treated rats. Our results suggested that the over expression of MLCK may be relatedwith the development of diabetic nephropathy.
Keywords: myosin light chain kinase; diabetes nephropathy; insulin; rats
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MDPI and ACS Style
Zhu, H.; Zhang, X.; Zuo, L.; Zhou, Q.; Gui, S.; Wei, W.; Wang, Y. Expression of Myosin Light Chain Kinase in Kidney of Streptozotocin-Induced Diabetic Rats. Int. J. Mol. Sci. 2006, 7, 510-518.
Zhu H, Zhang X, Zuo L, Zhou Q, Gui S, Wei W, Wang Y. Expression of Myosin Light Chain Kinase in Kidney of Streptozotocin-Induced Diabetic Rats. International Journal of Molecular Sciences. 2006; 7(11):510-518.
Zhu, Huaqing; Zhang, Xiaolin; Zuo, Li; Zhou, Qing; Gui, Shuyu; Wei, Wei; Wang, Yuan. 2006. "Expression of Myosin Light Chain Kinase in Kidney of Streptozotocin-Induced Diabetic Rats." Int. J. Mol. Sci. 7, no. 11: 510-518.