Abstract: Oxidative stress is implicated in HIV-infection. It has been suggested that plant antioxidants may offer protection from viral replication and cell death associated with oxidative stress in patients with HIV/AIDS. Because of inherent antioxidant properties of turmeric (T) and its derivatives, water-soluble extract turmerin (Tm) and lipid soluble curcumin (Cu), their potential efficacy as anti-HIV drugs were examined. Cell viability and p-24 antigen release by CEMss-T cells (1 x 105 cells/ml) infected with HIV-IIIB strain, used as an acute model of infection, were tested in the presence of 3’azido-3’deoxythmidine (AZT). Proliferative responses of human mononuclear cells derived from HIV patients (chronic model) stimulated with phyohemagglutinin (PHA), concanavalin A (ConA), and pokeweed mitogen (PWM) were also examined in the presence of AZT and Tm. In the infection assay, T, Tm and Cu individually did not reduce p-24 antigen release or improve cell viability. AZT (5μM) + Tm (800 ng/ml) inhibited infection by 37 % and increased cell numbers by 30%; whereas, Tm (80 ng/ml) inhibited infection by 26% and increased cell number by 60%. In the proliferation assay, lymphocytes from HIV-infected patients showed better inhibition of mitogen responsiveness to Tm (800 ng/ml) when compared to AZT at 5 μM or Tm at 80 ng/ml. Turmerin inhibited HIV-infected T-cell proliferation and, in combination with AZT, decreased T-cell infection and increased cell viability. These data provide evidence suggesting that efficacious anti-HIV therapy may be possible using lower, less toxic doses of AZT in the presence of turmerin.
Keywords: Turmeric; turmerin; curcumin; p-24 antigen; proliferation
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Cohly, H.H.P.; Asad, S.; Das, S.K.; Angel, M.F.; Rao, M. Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus. Int. J. Mol. Sci. 2003, 4, 22-33.
Cohly HHP, Asad S, Das SK, Angel MF, Rao M. Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus. International Journal of Molecular Sciences. 2003; 4(2):22-33.
Cohly, H. H.P.; Asad, S.; Das, S. K.; Angel, M. F.; Rao, M. 2003. "Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus." Int. J. Mol. Sci. 4, no. 2: 22-33.