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Effect of Turmeric, Turmerin and Curcumin on Ca2+, Na/K+ Atpases in Concanavalin A-Stimulated Human Blood Mononuclear Cells
Departments of Surgery and Medicine, University of Mississippi Medical Center, Jackson, MS, USA
Department of Biology, Alcorn State University, Lorman, MS, USA
* Author to whom correspondence should be addressed.
Received: 7 June 2002 / Accepted: 30 October 2002 / Published: 31 January 2003
Abstract: Ion transport enzymes may play an important role in T cell activation. This study investigates the role of turmeric and its individual components, turmerin-and curcumin-on Ca2+ and Na/K+ adenosine triphosphatases (ATPase) in the course of T cell activation. Concanavalin A (Con A) stimulated human blood mononuclear T cell proliferation paradigm was investigated for 3, 5 and 7 day periods with different concentrations of turmeric, curcumin and turmerin. Con A-stimulated cells treated with turmeric (250, 50, 5 μg/ml) for 3 and 5 days inhibited ATPase levels when compared to base levels obtained by cells in media alone. At day 7, there was a 3-fold increase for Ca2+ATPase levels and a 2-fold increase for Na/K+ATPase. Curcumin (250, 50, 5 μg/ml) showed the same pattern for ATPase activity as turmeric at 3 and 5 days with a 2-fold increase at day 7. Turmerin (2500, 1250, 250, 25 ng/ml) for Na/K+ ATPase activity showed an increase at day 3, a decrease on day 5, and a 2-fold increase on day 7. Ca2+ ATPase activity in the presence of turmerin showed an increase in ATPase levels at day 3 (except at 2500ng/ml where it decreased) and a decrease in day 5 (except at 25 ng/ml where it increased). Turmeric and curcumin generally inhibited Ca2+ATPase and Na/K+ATPases in early (day 3) and intermediate (day 5) stages of mitogen stimulation. However, the effect after 7 days incubation for turmeric, curcumin and turmerin showed a marked increase up to three fold.
Keywords: Turmeric; turmerin; curcumin; Ca2+ATPase; Na/K+ATPase
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Cohly, H.H.P.; Rao, M.-R.; Kanji, V.K.; Patlolla, B.; Taylor, A.; Wilson, M.T.; Angel, M.F.; Das, S.K. Effect of Turmeric, Turmerin and Curcumin on Ca2+, Na/K+ Atpases in Concanavalin A-Stimulated Human Blood Mononuclear Cells. Int. J. Mol. Sci. 2003, 4, 34-44.
Cohly HHP, Rao M-R, Kanji VK, Patlolla B, Taylor A, Wilson MT, Angel MF, Das SK. Effect of Turmeric, Turmerin and Curcumin on Ca2+, Na/K+ Atpases in Concanavalin A-Stimulated Human Blood Mononuclear Cells. International Journal of Molecular Sciences. 2003; 4(2):34-44.
Cohly, Hari H.P.; Rao, Maheshwara-Rajeswara; Kanji, Vijaya K.; Patlolla, Babu; Taylor, Anelle; Wilson, Melanie T.; Angel, Michael F.; Das, Suman K. 2003. "Effect of Turmeric, Turmerin and Curcumin on Ca2+, Na/K+ Atpases in Concanavalin A-Stimulated Human Blood Mononuclear Cells." Int. J. Mol. Sci. 4, no. 2: 34-44.